矛尾复虾虎鱼溃疡病病原创伤弧菌的鉴定  

Identification of Vibrio vulnificus from Synechogobius hasta with canker disease

作　　者：毕可然[1] 张晓君[1] 梁利国[1] 阎斌伦[1] 

机构地区：[1]淮海工学院江苏省海洋生物技术重点实验室,江苏连云港222005

出　　处：《海洋科学》2011年第7期20-25,共6页Marine Sciences

基　　金：江苏省水产三项工程资助项目(PJ2010-58);江苏省自然科学基金资助项目(BK2009163);江苏省生物多样性与生物技术重点实验室开放基金资助项目(164070302104)

摘　　要：2009年8月江苏连云港赣榆县多家虾蟹养殖塘混养的矛尾复虾虎鱼(Synechogobius hasta)大量死亡,从病鱼深层溃烂组织及肝脏中分离出大量优势生长的细菌,人工感染试验证明分离菌对虾虎鱼的半数致死量(LD50)为1.63×106 cfu/g。对分离菌进行了形态特征、理化特性和分子生物学等方面的分析。一方面,分离菌株16S rRNA和gyrB基因序列的BLAST搜索及最大简约法分析均表明其与创伤弧菌(Vibrio vulnificus)亲缘关系最近;另一方面,分离菌革兰氏阴性,氧化酶阳性,葡萄糖发酵型。因此,引起江苏连云港赣榆县矛尾复虾虎鱼溃疡病病原菌是弧菌属的创伤弧菌。In August 2009, mass mortality of Synechogobius hasta was observed in the shrimp and crabs pond of Ganyu county, Lianyungang city, Jiangsu province. Some pathogenic organisms were isolated from the liver and deep ulceration of the diseased S. hasta. The dominant bacterial strain was Gram-negative, oxidase positive, and fermented glucose to produce acids. In addition, 16S rRNA and gyrB genes analysis showed that isolation was closely related to Vibrio vulnificus than other bacterium. The sequences similarity coefficients were 0.99, on the other hand, and the phylogenetic trees strongly supported isolation were grouped with some reported V. vulnificus. Moreover, the LD50 of the bacteria to S. hasta was 1.63 ×10^6cfu/g. We concluded that V.vulnificus was an important pathogenic bacteria causing canker disease in S. hasta in Ganyu county, Lianyungang city, Jiangsu province.

关 键 词：矛尾复虾虎鱼(Synechogobius hasta) 创伤弧菌(Vibrio vulnificus) 16S RRNA基因 gyrB基因 

分 类 号：S968[农业科学—水产养殖]