海南地区溶藻弧菌遗传多样性的RAPD分析  

RAPD analysis of genetic diversity of Vibrio alginolyticus isolated from Hainan province

作　　者：欧阳吉隆[1] 周永灿[1] 吴学贵[1] 戴小连[1] 王世锋[1] 谢珍玉[1] 

机构地区：[1]海南大学海南省热带水生生物技术重点实验室,海南海口570228

出　　处：《海洋科学》2011年第7期30-36,共7页Marine Sciences

基　　金：国家科技支撑计划项目(2009BAB44B02);国家自然科学基金项目(30760190;31060360);霍英东教育基金会高等院校青年教师基金基础性研究课题(121030);农业科技成果转化项目(2009GB2E200302)

摘　　要：以来源于泰国的菌株TG06003作对照，对采自海南地区的27株溶藻孤菌（Vibrio alginolyticus）环境菌株进行了随机扩增多态性DNA标记（RAPD）分析，其中6株为毒力菌株，另外22株为非毒力菌株。实验共得到70个多态性条带，多态性比率（PP13）为98．6％。基因多样度的算术平均数和加权平均数分别为0．922和0．4544，Shannon’s信息指数的算术平均数和加权平均数分别为0．1391和0．1713。遗传相似度为0．4429-0．8857，遗传距离为0．1214-0．7520。将溶藻弧菌分为毒力菌株和非毒力菌株两个类群进行分析，结果表明，毒力菌株的遗传多样性丰富，它们的基因多样度和Shannon’s信息指数明显高于非毒力菌株；毒力菌株HN08811、HN08155、HN08809、HN08813明显聚为一类，另外2株毒力菌株分别与非毒力菌株HN08304和HN08803聚为一类，溶藻弧菌毒力菌株存在多种遗传型。Taking the Thai strain TG06003 as a control, 27 strains of Vibrio alginolyticus collected from Hainan maricultural environment were analyzed using the random amplified polymorphic DNA marker （RAPD） method. Six strains were virulent and the other 22 strains were non-virulent. Seventy polymorphic bands were produced by PCR with eight selected primers, and the PPB was 98.6%. The arithmetic average and the weighted average of gene diversity were 0.922 and 0.454 4, respectively, and the arithmetic average and the weighted average of Shannon＇s information index were 0.1391 and 0.171 3, respectively. Distribution of genetic similarity was between 0.442 9 0.885 7, and genetic distances were distributed between the 0.121 4-0.752 0. Virulent strains HN0881 l, HN08155, HN08809, and HN08813 were clearly clustered together. However, the other two virulent strains were clustered with non-virulent strains. The gene diversity of virulent strains was high, and their genetic diversity and Shannon＇s information index were significantly higher than those of non-virulent strains. Our results indicate that virulent strains and non-virulent strains of Vibrio alginolyticus have multiple genetic types.

关 键 词：溶藻弧菌(Vibrio alginolyticus) RAPD 毒力菌株 遗传多样性 

分 类 号：S941.42[农业科学—水产养殖]