高致病性猪繁殖与呼吸综合征病毒感染仔猪外周血细胞的动态变化  

Dynamic changes of blood cells from piglets infected with high pathogenic porcine reproductive and respiratory syndrome virus

作　　者：孙广力[1,2] 蔡雪辉[3] 郑世民[1] 刘永刚[3] 孙刚 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030 [2]黑龙江省动物卫生监督所,黑龙江哈尔滨150069 [3]中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨150001

出　　处：《中国兽医杂志》2011年第7期13-15,共3页Chinese Journal of Veterinary Medicine

基　　金：黑龙江省自然科学基金项目(C200841)

摘　　要：目的是通过检测HP-PRRS仔猪外周血细胞数量,探讨HP-PRRSV致病机制。方法是以HuN4株感染60日龄长白仔猪,应用分子生物学技术、全自动血液分析仪,对感染PRRSV仔猪外周血病毒载量、血细胞数量进行检测。结果显示PRRSV感染仔猪外周血病毒载量超过103copies/mL,WBC显著下降(P<0.05),RBC、HGB、HCT表现出周期性减少和增多(P<0.05),PLT显著下降(P<0.05);相关性分析表明,PRRSV载量对数值与WBC、PLT呈现中度负相关(0.40<r<0.69),与HGB、MCH存在低度负相关(0.20<r<0.39)。表明HP-PRRS仔猪外周血细胞改变可能与PRRSV持续感染、免疫抑制、局部和全身血液循环障碍密切相关。This study was to investigate the changes of peripheral blood cells of piglets with high pathogenic porcine reproductive and respiratory syndrome（HP-PRRS）.The viral loads and blood routine of peripheral blood were tested in groups of 60 days old Chang-Bai piglets inoculated by PRRSV HuN4 strain.Results The virus was detected from serum of piglets at each sample collection period through the end of the trial,and the amounts of PRRSV RNA ranged over 103 copies/mL.Compared with the results of control groups,the levels of WBC were significantly lower（P0.05）,the levels of RBC,HGB,HCT were significantly lower（P0.05） and then significantly higher（P0.05）,the levels of PLT was significantly lower（P0.05）.The log values of PRRSV load in serum of principal piglets had moderate negative correlation with the levels of WBC and PLT（0.40r0.69）,and had weak or low negative correlation with the levels of HGB and MCH（0.20r0.39）.The results from the examination of the blood samples from HP-PRRS piglets changed significantly,suggesting that HP-PRRS has persistent infection,immune suppression,local and general circulatory disturbances of the blood.

关 键 词：高致病性猪繁殖与呼吸综合征 血细胞 

分 类 号：S852.659.1[农业科学—基础兽医学]