注射酮色林对脊神经结扎大鼠脊髓背角和背根神经节nNOS表达的影响  

Effect of Injecting Ketanserin on Expression of nNOS in Spinal Dorsal Horn and Dorsal Root Ganglin in Spinal Nerve Ligation Rats

作　　者：林敏捷[1] 王冬梅[1] 洪炎国[1] 

机构地区：[1]福建师范大学生命科学学院,福建省发育与神经生物学重点实验室,福建福州350108

出　　处：《福建师范大学学报（自然科学版）》2011年第4期117-121,共5页Journal of Fujian Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(30570600;30970985)

摘　　要：通过结扎大鼠L5脊神经建立神经病理性痛模型.手术后第3日开始皮下注射酮色林(0.3 mg.kg-1)或其溶剂DMSO(体积分数为0.8%),每d 1次,2周后灌流、取材,免疫组织化学实验显示神经结扎后L4背根神经节(DRG)和脊髓背角-层nNOS的表达均上调.然而皮下注射酮色林阻断5-HT2A受体,使L4 DRG和脊髓背角细胞中nNOS的表达大大减少.说明阻断外周5-HT2A受体后,能抑制神经病理性痛DRG和脊髓背角nNOS的表达上调.Neuropathic pain model was established by L5 spinal nerve ligation（SNL） in rats.Vehicle（0.8% DMSO） or ketanserin（0.3 mg·kg-1） was injected subcutaneously once per day starting on day 3 following the surgery for two weeks.Immunohistochemistry study revealed that the expression of neuronal nitric oxide synthase（nNOS） in L4 dorsal root ganglion（DRG） and NADPH-d-stained neurons in laminae Ⅰ-Ⅱ of the spinal cord dorsal horn were greatly increased following the surgery of SNL.However,injection of the 5-HT2A receptor antagonist ketanserin,but not vehicle,completely abolished the increase in nNOS or NADPH-d expression in the spinal cord and DRG.The results in present study suggest that blockade of 5-HT2A receptor in the periphery inhibited upregulation of nNOS at the spinal level.

关 键 词：5-HT2A受体 酮色林 神经病理性疼痛 背根神经节 脊髓 一氧化氮合成酶 

分 类 号：Q426[生物学—神经生物学]