抗虫基因在湘杂棉22号F_1与F_2代中的表达及遗传稳定性研究  

Expression and Genetic Stability of Insect-resistant Gene in F_1 and F_2 of Xiangzamian No.22

作　　者：曾潜[1,2] 张学文[1] 彭珍子[1] 黄丽华[1] 陈浩东[2] 戴恺[2] 

机构地区：[1]湖南农业大学生物科技学院,湖南长沙410128 [2]湖南省棉花科学研究所,湖南常德415101

出　　处：《湖南农业科学》2011年第7期5-7,10,共4页Hunan Agricultural Sciences

基　　金：湖南省农业厅2007~2010年科技支撑计划资助

摘　　要：以转基因抗虫棉湘杂棉22号为研究材料,利用PCR和ELISA检测方法,分析了Cry1Ac基因与NptⅡ基因在F1与F2代材料中的表达及遗传稳定性情况。结果显示,外来抗虫基因在F1代中稳定存在,且稳定表达。F2代检测样本中5%的个体出现基因沉默,表达个体中的表达量存在差异。Cry1Ac基因与NptⅡ基因呈现紧密连锁关系,检测样本中仍有2.4%的个体发生了分离。该结果结合其丰产性可为定株选择稳定转基因抗虫育种材料提供依据。Using detection methods of PCR and ELISA to analyze the expression and genetic stability of NptⅡ gene and Cry1Ac gene in F1 and F2 of transgenic insect-resistant cotton Xiangzamian No.22.The results showed that foreign insect-resistant genes in F1 were stable existing and expression;5% of individuals appeared gene silencing in F2 samples,and the expressed individuals were different in the expression amount;Cry1Ac gene showed close linkage relation with Npt Ⅱ gene,but 2.4% of individuals were separated in samples.These results combined with high yielding ability of Xiangzamian No.22 will provide the basis for selecting breeding materials with stable transgenic insect-resistance.

关 键 词：转基因抗虫棉 湘杂棉22号 CRY1AC基因 NptⅡ基因 ELISA检测 遗传稳定性 

分 类 号：S562[农业科学—作物学]