光暗交替及全黑暗对水杉种子萌发过程中同工酶的影响  

Influences of Alternation of Light and Darkness and Full Darkness on Isozymes in Germination Process of Metasequoia glyptostroboide Seeds

作　　者：景丹龙[1] 王玉兵[1] 梁宏伟[1] 王静 张德春[1] 

机构地区：[1]三峡大学生物技术研究中心,湖北宜昌443002 [2]神农架国家级自然保护区管理局生物多样性保护研究实验室,湖北木鱼442400

出　　处：《湖南农业科学》2011年第7期48-50,共3页Hunan Agricultural Sciences

基　　金：国家自然科学基金(30670202);湖北省生物资源保护与利用重点实验室开放基金(2007010);宜昌市科技重点攻关项(A06209)

摘　　要：用非变性聚丙烯凝胶电泳技术对光暗交替和全黑暗条件下水杉种子的酯酶(EST)、过氧化物酶(POD)、淀粉酶(AMY)、超氧化物歧化酶(SOD)和ATP酶(ATPase)5种同工酶进行分析。结果表明:水杉种子的5种同工酶含量在光暗交替条件下明显优于全黑暗条件。其中,光暗交替条件下的5种同工酶与全黑暗条件相比,EST酶带数多,SOD和POD同工酶酶带数提前达到最多,且各时期的AMY和ATPase含量更高。证实光暗交替条件更利于水杉种子的萌发。Non-denaturing polyacrylamide gel electrophoresis was used to analyze five kinds of isozyme,such as esterase（EST）,peroxidase（POD）,amylase（AMY）,superoxide dismutase（SOD） and ATPase,in Metasequoia glyptostroboides seeds under alternation of light and darkness or full darkness.The results showed that the contents of five isozymes in Metasequoia glyptostroboides seeds under alternation of light and darkness were higher than that under all darkness;in comparison with full darkness,the five isozymes under alternation of light and darkness had more band numbers of EST isozyme,the maximum band numbers of SOD and POD isozymes in advance,and the higher contents of AMY and ATPase in each period.Therefore,the alternation of light and darkness is more beneficial to the germination of Metasequoia glyptostroboide seeds.

关 键 词：光暗交替 全黑暗 水杉种子 同工酶 萌发 

分 类 号：S791.35[农业科学—林木遗传育种]