四个绵羊品种MHC-DQA1基因exonⅡ多态性分析  

Polymorphisms of MHC-DQA1 Gene exonⅡ in Four Sheep Breeds

作　　者：李敏 任艳玲 肖娜 董文艳 沈志强 

机构地区：[1]山东绿都生物科技有限公司,山东滨州256600 [2]山东滨州畜牧兽医研究院,山东滨州256600 [3]山东省洼地绵羊繁育技术研究推广中心,山东滨州256600

出　　处：《动物医学进展》2011年第7期29-32,54,共5页Progress In Veterinary Medicine

基　　金：国家高技术研究发展计划(863计划)项目(2008AA10Z142);山东省自然科学基金项目(Y2007D12);山东省良种工程项目(2006GG2209028)

摘　　要：为了比较和揭示洼地绵羊、杜泊羊、小尾寒羊及特克赛尔绵羊4个绵羊品种MHC-DQA1基因的遗传多态性,采用PCR-RFLP技术对4个绵羊品种224个样本的MHC-DQA1基因exonⅡ的315 bp片段进行多态性分析;并对基因型频率和等位基因频率进行统计,运用Popgen32软件计算相应的遗传参数。结果表明,AluI-RELP检测的4个绵羊品种224只群体中,出现3种基因型MM、MN、NN,其中N为优势等位基因,且MM基因型只分布于洼地绵羊中。遗传参数分析表明,除小尾寒羊外,洼地绵羊、杜泊羊及特克赛尔羊AluI-RELP位点杂合度均高于纯合度;且洼地绵羊表现中度多态,其他绵羊品种表现低度多态。4个绵羊品种224只群体MHC-DQA1基因exonⅡ在AluI-RELP位点具有较低的遗传多样性。The polymorphisms of MHC-DQA 1 gene exonⅡ in four sheep breeds（Wadi sheep,Dorper sheeps,small tailed han sheeps and exel sheep） were analyzed.PCR-RFLP method was used to study the genetic polymorphisms of 315 bp MHC-DQA 1 gene exonⅡ in 224 samples of four sheep breeds,then the genotypic frequences and allelic frenquency were analyzed.Software Popgen32 was employed to calculate the relative genetic parameters.The results of AluI-RELP showed that there are two allele M and N at AluⅠ-RFLP locus in 224 samples of four sheep breeds,and by comparing with the allelic frequency,the predominant allele is N;The results of genetic parameters showed that except for small tailed han sheep,the heterozygosity are higher than homogeneity at AluⅠ-RFLP locus in other three sheep breeds;and the results of genetic parameters showed that there were low polymorphism in exon 2 of MHC-DQA1 gene in Dorper sheep,small tailed han sheep and Texel sheep,while it was moderate polymorphism in Wadi sheep.There were low genetic diversities in exonⅡ of MHC-DQA1 gene at AluⅠ-RFLP locus in 224 samples of four Sheep breeds.

关 键 词：洼地绵羊 杜泊羊 小尾寒羊 特克赛尔 MHC-DQA1 PCR-RFLP 遗传多态性 

分 类 号：Q789[生物学—分子生物学]