牛乳腺上皮细胞体外分离和培养  

Study on Culture of Bovine Mammary Epithelial Cells in Vitro

作　　者：弓超[1] 王凌云[1] 周欢敏[1] 

机构地区：[1]内蒙古农业大学生命科学学院,内蒙古呼和浩特010018

出　　处：《动物医学进展》2011年第7期66-71,共6页Progress In Veterinary Medicine

基　　金：内蒙古生物高科技资助项目

摘　　要：从健康奶牛乳房无菌采取乳腺组织,通过添加两种不同的培养液来分离、培养、纯化牛乳腺上皮细胞,研究乳腺上皮细胞的体外培养效果。结果表明,使用组织块接种可以得到大量细胞用于体外培养。在以DMEM/F12为基础的普通培养液中进行乳腺上皮细胞体外培养,原代细胞生长较慢,细胞形态不典型。而添加表皮生长因子、胰岛素、氢化可的松所组成的完全培养液中,奶牛乳腺上皮细胞生长良好。并通过细胞形态学观察,细胞染色体核型分析,荧光免疫细胞染色方法鉴定了培养的细胞表达上皮细胞特异的角蛋白K14,K15。结果表明,分离培养的细胞是牛乳腺上皮细胞。In this research,bovine mammary epithelial cells（BMEC） were isolated,purified and cultured in vitro,and further characterized by morphological observation and karyotyping analysis.The tissue-specific expression of cytokeratin K14 and K15 in BMEC was also identified by immunofluorescent cytochemical staining.The results showed that the large number of BMEC could be separated from mammary tissue inoculation.Complete growth medium can cultured BMEC better than DMEM/F12.All these results demonstrated that the purified cells were BMEC.

关 键 词：乳腺上皮细胞 体外培养 奶牛 

分 类 号：Q813.11[生物学—生物工程]