基于抗病毒活性检测的板蓝根质量生物评价方法及优化研究  

Bio-evaluation methods and optimization for Isatidis Radix quality control based on antiviral activity detection

作　　者：李寒冰[1] 鄢丹[2] 武彦舒[3] 肖小河[2] 唐慧英[2] 张少峰[2] 

机构地区：[1]河南中医学院药学院临床药理研究室,河南郑州450008 [2]解放军中药研究所解放军302医院,北京100039 [3]榆林市第二医院药剂科,陕西榆林719000

出　　处：《中草药》2011年第8期1560-1565,共6页Chinese Traditional and Herbal Drugs

基　　金：国家杰出青年科学基金项目(30625042);国家重大新药创制专项课题资助项目(2009ZX09502-022);河南中医学院博士科研基金(BSJJ2009-09)

摘　　要：目的建立基于抗病毒活性检测的板蓝根质量生物评价方法并进行优化研究。方法分别采用红细胞凝集活性检测法和流感病毒神经氨酸酶(NA)活性检测法,建立板蓝根抗病毒活性生物测定方法,并对所建立的两种方法进行对比分析与优选。结果两种方法可用于对不同样品进行活性检测和区分,结果重复性较好(RSD=7.0%、5.78%);Pearson相关性分析表明板蓝根凝集活性与NA抑制活性之间具有显著相关性(P<0.01,r=?0.81),进一步印证了凝集活性测定法与抗病毒药理作用的关联性;在安全性、经济性、简便性和实用性等方面凝集活性检测法优势明显,可作为板蓝根质量生物测定的优选方法。结论本研究优选凝集活性检测法用于板蓝根质量的生物测定,与抗流感病毒药效相关,提高了其现行质量控制水平,并为中药质量生物测定方法的研究提供参考。Objective To establish and optimize a bio-evaluation method based on antiviral activity detection for lsatidis Radix quality control. Methods According to the fact that antivirus activity is the main pharmacological effect of lsatidis Radix, the means of hemagglutination activity detection and the means of influenza virus neuraminidase （NA） activity detection were used for establishing two antiviral activity detection methods for the bio-evaluation oflsatidis Radix. In addition, contrastive analyzation and optimization of the two methods were carried out in this study. Results The two methods could be used to evaluate and distinct the biological activities of different batches oflsatidis Radix samples and both methods have good repeatabilities （RSD = 7.0% and 5.78%）. The result shows a good correlation （P〈0.0I, r = -0.81） between the neuraminidase inhibitory activity and the hemagglutination activity of Isatidis Radix. The relationship between agglutination activity and antiviral effect of lsatidis Radix was further verified. Agglutination detection method has the superiority to its safety, inexpensiveness, easiness, and practicality. Conclusion This novel method could reflect the pharmacodynamic activity of lsatidis Radix on anti-influenza virus and be helpful for the quality control oflsatidis Radix. This study provides reference for the bio-evaluation on the quality control of Chinese materia medica.

关 键 词：板蓝根:质量控制 生物评价 抗病毒 活性检测 

分 类 号：R285.5[医药卫生—中药学]