康复训练对脊髓损伤大鼠外源性移植的骨髓源性神经干细胞的影响  

Experimental study of the effects of rehabilitation training on transplanted BMSC-D-NSCs in rats with spinal cord injury

作　　者：周治来[1] 陈银海[2] 闵少雄[1] 

机构地区：[1]南方医科大学珠江医院骨科中心,广州市510282 [2]南方医科大学珠江医院门诊部,广州市510282

出　　处：《实用医学杂志》2011年第17期3103-3106,共4页The Journal of Practical Medicine

基　　金：广东省自然科学基金资助项目(编号:8451051501000460)

摘　　要：目的:研究康复训练对移植到脊髓损伤大鼠体内的骨髓源性神经干细胞的存活、迁移、分化以及大鼠功能恢复的影响。方法:制作40只T10脊髓损伤动物模型,随机分成4组,分别为移植组(Y组)、康复训练组(K组)和康复训练联合细胞移植组(L组)、对照组(C组),在脊髓损伤后第7天将培养并标记好的骨髓源性神经干细胞移植到Y、L组中。分别在脊髓损伤后1、3、7d及2、3、4、5、6周对所有动物进行BBB评分。免疫荧光染色观察移植的细胞在脊髓内的迁移、分化的情况。结果:BBB评分显示,细胞移植2周后,L组评分明显高于其他各组(P<0.01)。免疫荧光染色显示,L组动物的神经干细胞存活率较Y组好,分化为神经元的比例更高。结论:康复训练能够促进移植的神经干细胞在脊髓中的存活并能促进其分化,有益于脊髓损伤大鼠后肢功能的恢复。Objective To explore the effects of rehabilitation training on promoting transplanted BMSC-D- NSCs migration and differentiation in rats with spinal cord injury. Method 40 Sprague-Dawley rats those were experienced modified Allen impactor（H = 25 mm）at T10 , then were randomly divided into 4 groups averagely： a transplantation group （group Y）, a rehabilitation training group （group K）, a combined group （group L）,and a control group （group C）. At the 7th day post SCI, BrdU labeled ceils were transplanted into the injured spinal cord of rats in group Y and group L, The hindlimbs movement was assessed by BBB scales ; immunofluorescence staining were used to detect BMSC-D-NSCs migration and differentiation. Results The BBB scores of rats in group L was significant higher than those in other groups since 2 weeks after operation（P 〈 0.01 ） ;Immunofluorescence staining showed that the survival and differentiation of grafted cell in group L were better than those in group Y. Conclusions rehabilitation training can promote the survival and differentiation of the transplanted BMSC-D-NSCs in the injured spinal cord, and play a role in restore the motor function of rats with SCI.

关 键 词：脊髓损伤 康复训练 骨髓源性神经干细胞 细胞移植 

分 类 号：R651.2[医药卫生—外科学]