银翘散对感染甲1型流感病毒金刚烷胺耐药株小鼠的抗病毒研究  

Experimental study on the action of Lonicerae and Forsythiae powder against amantadine-resistant influenza A virus(H1N1) in vivo

作　　者：关文达[1] 杨子峰[1,2] 谢慧珺[3] 赵瑾[1] 王玉涛[1] 秦笙[1] 招穗珊[1] 伍时冠[1] 潘思华[1] 陈俏妍[4] 

机构地区：[1]广州医学院第一附属医院呼吸疾病国家重点实验室,广东广州510120 [2]澳门科技大学中医药学院,澳门特别行政区00853 [3]广州中医药大学热带医学研究所,广东广州510405 [4]广东省中医院耳鼻喉科,广东广州510120

出　　处：《广东农业科学》2011年第13期135-137,F0003,共4页Guangdong Agricultural Sciences

基　　金：广东省中医药局建设中医药强省科研课题(2009425);广东省教育厅;财政厅高校学科专业建设与质量工程专项基金高水平科研项目(粤财教[2010]275号);广州市卫生局中医药;中西医结合科研课题(穗卫中[2009]4号);广州中医药大学科研创新基金(10CX21)

摘　　要：以经过耐药表型和基因型鉴定的甲1型流感病毒金刚烷胺耐药株感染BALB/c小鼠,观察其生存状态并测定肺指数,以苏木素—伊红染色(HE染色)法观察肺组织病理改变;将鼠肺悬液以空斑试验定量检测感染性病毒滴度。研究结果表明,银翘散可以减轻甲1型流感耐药株感染小鼠引起的全身症状,降低肺指数;减少鼠肺病变范围及肺泡渗出物;降低鼠肺病毒滴度。说明银翘散有体内抑制甲1型流感病毒金刚烷胺耐药株复制的作用。The antiviral effects of Lonicerae and Forsythiae Powder were observed in vivo,and the impacts on living states,lung index,virus titer of lungs,pathological changes of lungs were observed in the mice infected with amantadine-resistant influenza a virus.The results showed that Lonicerae and Forsythiae Powder significantly inhibited the lung consolidation of mice pneumonia caused by the infection of influenza virus and replication of the infectious viral particles in lungs at the concentration.No significant antiviral effect of Lonicerae and Forsythiae Powder at the concentration of inhibiting amantadine-resistant influenza a virus was observed.It was concluded that Lonicerae and Forsythiae Powder was effective in inhibiting anti-amantadine-resistant influenza virus A（H1N1） in vivo.

关 键 词：银翘散 流感病毒 体内 耐药 

分 类 号：R285.5[医药卫生—中药学]