鸡Nramp1基因多态性与部分免疫指标相关性分析  

Correlation between Polymorphisms of Nramp1 Gene and Immune Traits in Chicken

作　　者：胡国顺[1] 常国斌[1] 王克华[2] 刘艳[1] 陈蓉[1] 栾德琴[1] 龚琳琳[1] 陈国宏[1] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]中国农科院家禽研究所,江苏扬州225003

出　　处：《中国畜牧杂志》2011年第15期5-8,共4页Chinese Journal of Animal Science

基　　金：江苏省科技支撑计划(BE2010375);江苏省科技支撑计划(BE2008359);江苏省自然科学基金(BK2009190)

摘　　要：随机选取同期孵化出雏的中国地方鸡种如皋鸡和引进隐性白羽鸡72只和55只,测定部分免疫性状。利用PCR-SSCP技术检测如皋鸡和隐性白羽鸡Nramp1基因第9外显子的多态性,并对不同基因型与免疫性状进行相关分析。结果表明:如皋鸡与隐性白羽鸡H/L、淋巴细胞转化率和IgM含量均差异显著(P<0.05)。如皋鸡AA型的H/L显著低于BB型和AB型(P<0.05),而AA型的淋巴细胞转化率和IgM含量显著高于BB型(P<0.05)。隐性白羽鸡各基因型对免疫指标的影响与如皋鸡的趋势相同。该研究结果初步显示,如皋鸡的综合免疫性能优于隐性白羽鸡,AA基因型的综合免疫性能优于AB型和BB型。A total of 72 Rugao chicken（RG） and 55 Recessive White chicken（RW）,which belong to Chinese native chicken breeds and introduced breeds respectively,were selected randomly and incubated at the same stage.The value of immune traits was detected.PCR-SSCP technique was applied to analyze the correlation between the polymorphisms of Nramp1 gene and immune traits in RG and RW.The results showed thatthe heterophil/lymphocyte（H/L） value,lymphocyte transformation rate and the content of IgM of RG and RW were significantly different（P0.05）.H/L value of AA genotype was significantly lower than that of BB genotype and AB genotype,while the AA genotype＇s lymphocyte transformation rate and IgM were significantly higher than those of the BB genotype in both RG and RW.The results demonstrated that the general immune performance of RG was superior to that of RW.The general immune performance of AA genotype was superior to that of BB genotype and AB genotype.

关 键 词：Nramp1基因 单核苷酸多态性 免疫性状 鸡 

分 类 号：S831.2[农业科学—畜牧学]