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作 者:权富生[1,2] 马会民[1] 张涌[1,2] 朱士恩[3,2] 周艳华[3,2]
机构地区:[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]现代农业(肉牛)产业技术体系遗传育种与繁殖功能研究室,北京100193 [3]中国农业大学动物科技学院,北京100193
出 处:《中国畜牧杂志》2011年第15期19-22,共4页Chinese Journal of Animal Science
基 金:现代农业(肉牛)产业技术体系项目(MATS-Beef Cattle System);国家支撑项目(2008BADB2B03)
摘 要:选择100头红安格斯母牛使用2种超数排卵处理方案,有效超排供体母牛77头。对受体牛采用3种方案进行同期发情处理。结果表明:育成母牛和经产母牛平均回收胚胎数分别为8.45、9.23枚(P>0.05),平均回收可用胚胎数分别为6.33、7.02枚(P>0.05);2种超排方案均取得较好的效果,超排方案1和方案2分别回收胚胎数为10.10、7.63枚,回收可用胚胎数为6.62、6.51枚。受体同期发情处理一次PG法、二次PG法及CIDR+PG法,同期发情率分别为71.19%、74.44%和77.63%(P>0.05),发情受体利用率分别为66.67%、64.93%及69.49%(P>0.05),胚胎移植妊娠率分别为50.00%、55.17%和56.10%(P>0.05),与自然发情受体利用率及胚胎移植妊娠率无显著性差异。One hundred red Angus cows were selected as donors of MOET nuclear breed herd,two protocols were designed,and 77 donors were effectively superovulated by using CAS(Chinese Academy of Science) FSH.Meanwhile,synchronization of estrus were induced for recipient by adopting 3 protocols.The results showed that the total embryos recovered from cows and heifers were 8.45 and 9.23,respectively,and the transferable embryo numbers were 6.33 and 7.02 respectively.The total number of embryos were 10.10 and 7.63 and the transferable embryos were 6.62 and 6.51 recovered by protocol 1 and protocol 2.The percents of estrus synchronization were 71.19%,74.44% and 77.63% respectively,the percents of recipient utilization were 66.67%,64.93% and 69.49%,and the pregnancy rate of recipient were 50.00%,55.17% and 56.10% by injecting PGF2α once or twice and the treatment by CIDR + PGF2α to induce estrus synchronization.The results indicated that good superovulation had been obtained by both protocol 1 and protocol 2 on red Angus cows and heifers.There were no significant effects on superovulatory between heifers and adult cows.Effects of synchronization of estrus were the same in three different protocols by luteal choice,and there were no differences between the natural estrus recipients and the introduced estrus recipients in utilization rate and pregnancy rate of recipient.
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