姜黄素对阿尔茨海默双转基因小鼠海马超微结构的影响  

Influence of curcumin on hippocampus ultrastructure in APPswe/PS1dE9 double transgenic mice

作　　者：王蓬文[1,2] 李瑞晟[1,3] 王虹[1] 任映[1] 孙海芸[1] 杨金铎[1] 朱志慧[1] 孙建宁[2] 

机构地区：[1]北京中医药大学东直门医院中药药理学实验室,北京100700 [2]北京中医药大学中药学院中药药理学实验室 [3]新疆医科大学第二附属医院老年病科

出　　处：《北京中医药大学学报》2011年第7期465-470,共6页Journal of Beijing University of Traditional Chinese Medicine

基　　金：北京市自然基金资助项目(No.7092057);国家自然基金资助项目(No.81073076)

摘　　要：目的应用电子显微镜,观察β-淀粉样前体蛋白swe基因/早老蛋白1dE9基因(APPswe/PS1dE9)双转基因小鼠海马CA1区神经元以及突触的变化,评价姜黄素对APPswe/PS1dE9双转基因小鼠认知功能的影响。方法将3月龄的APPswe/PS1dE9双转基因小鼠随机分为模型组、罗格列酮组(0.92 mg/kg)、姜黄素大、中、小剂量组(剂量分别为400、200、100 mg/kg),每组3只;并以同月龄遗传背景相同的C57BL/6 J小鼠作为正常对照组。每天灌胃给药1次,连续灌胃3个月。应用透射电子显微镜,检测海马超微结构变化。结果正常对照组海马神经元和神经毡内结构均正常。模型组小鼠海马CA1区神经元固缩、电子密度升高,胞浆内脂褐素、脂滴明显增多,海马CA1区神经元出现程度不等的退行性变;神经毡内可见髓鞘变性,突触数量减少、结构异常,突触小泡聚集增多。姜黄素可使突触增加,并使上述变化有不同程度的改善。结论姜黄素能通过增加突触、改变APPswe/PS1dE9双转基因小鼠突触等超微结构的变化,进而改善APPswe/PS1dE9双转基因小鼠的学习记忆能力。Objective To observe the changes of neurons and synapses in hippocampus CA1 zone in amyloid protein precursor （ APPswe ） / （ presenilin （ PS1 dE9 ） double transgenic mice with transmission electron microscope （ TEM）, and review the influence of curcumin on the cognitive function of APPswe/ PSl dE9 double transgenic mice. Methods All APPswe/PSldE9 double transgenic mice （3 months old） were randomly divided into the mode group, rosiglitazone （0. 92 mg/kg） group, and high-dose （400 mg/kg）, mid-dose （200 mg/kg） and low-dose （100 mg/kg） curcumin group （each n = 3）, and C57BL/6J mice with the same age and genetic background were assigned into the normal control group. All groups, except the normal control group, were intragastrically given relevant medicinal respectively once a day for 3 months. The changes of hippocampus ultrastructure were observed with TEM. Results The structure of hippocampus neutrons and neuropils were normal in the normal control group. In the model group, neuron pyknosis, higher electron density, increase of lipofuscin （LPF） and lipid droplets in cytoplasm, and degeneration of neutrons in varying degrees were observed in the hippocampus CA1 zone, and myelinic degeneration, decrease and structural abnormality of synapses, and increased aggregation of synaptic vesicles were observed in neuropils. Curcumin increased the number of synapses and ameliorated the above mentioned changes. Conclusion Curcumin can memory abilities in APPswe/PSI dE9 double transgenic mice through increasing improve the learn and the number of synapses and changing hippocampus ultrastructure.

关 键 词：姜黄素 β-淀粉样前体蛋白swe基因/早老蛋白1dE9基因双转基因小鼠 电镜 突触 

分 类 号：R285.5[医药卫生—中药学]