紫外分光光度法测定葛根黄豆苷元自微乳中药物的含量  

Content Determination of Daidzein Self-microemulsifying Agent by UV Spectrophotometry

作　　者：张娜[1] 张娜1 康虞玲[1] 杨更亮[1] 

机构地区：[1]河北大学药学院/河北省质量分析控制重点实验室,保定市071000

出　　处：《中国药房》2011年第31期2927-2928,共2页China Pharmacy

基　　金：河北省高等学校自然科学研究指导计划项目(Z2010120)

摘　　要：目的:建立测定葛根黄豆苷元自微乳中药物含量的方法。方法:采用紫外分光光度法,在250nm波长处直接测定葛根黄豆苷元自微乳中药物的含量。结果:葛根黄豆苷元的检测浓度在0.5～7.0mg.L-1范围内与吸光度呈良好线性关系(r=0.9995);平均加样回收率为99.27%,RSD=0.64%(n=9)。结论:本方法简便、快速、结果准确,可用于自微乳药物制剂的快速分析。OBJECTIVE：To develop a method for the content determination of daidzein self-microemulsifying agent.METHODS：The content of daidzein self-microemulsifying agent was determined directly at 250 nm by UV spectrophotometry.RESULTS：The calibration curve for daidzein was linear within the range of 0.5～7.0 mg·L-1（r=0.999 5）with an average recovery rate of 99.27%（RSD=0.64%,n=9）.CONCLUSION：This method is simple,rapid and accurate,and suitable for the quality control of self-microemulsifying agent.

关 键 词：葛根黄豆苷元 自微乳化药物传递系统 紫外分光光度法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]