我国南方捕鸟蛛一新种的生物化学鉴定(蜘蛛目,捕鸟蛛科)  

Biochemical Identification of Selenocosmia hainana sp.nov.from South China (Araneae, Theraphosidae)

作　　者：梁宋平[1] 彭贤锦[1] 黄仁槐[1] 陈平[1] 

机构地区：[1]湖南师范大学生命科学学院,中国湖南长沙410081

出　　处：《生命科学研究》1999年第4期299-304,共6页Life Science Research

基　　金：国家自然科学基金!(39370152)

摘　　要：采用高效液相色谱（ＨＰＬＣ）、激光解析基质辅助电离飞行时间质谱（ＭＡＩＤＩ－ＴＯＦＭＳ）和蛋白质序列分析方法，对从我国海南通什地区发现的一种捕鸟蛛与广西虎纹捕鸟蛛（Ｓｅｌｅｎｏｃｏｓｍ ｉａｈｕｗ ｅｎａ）的毒液进行了比较分析． 虽然两种蜘蛛形态十分相似，但其毒液的化学组成和主要毒素的氨基酸序列存在明显的差异，说明两种蜘蛛在进化上有同源性但分化很早．应列为不同种，特将海南发现的蜘蛛新种定名为海南捕鸟蛛，新种Ｓｅｌｅｎｏｃｏｓｍ ｉａ ｈａｉｎａｎａ ｓｐ．ｎｏｖ．By means of reverse phase and ion exchange high performance liquid chromatography (HPLC), matrix assisted laser desorption/ionization time of flight mass spectrometer (MAIDI TOF MS) and amino acid sequence analysis of peptides, the venom from the spider found in Tongshi county, Hainan province of south China was analyzed and compared with that of the spider Selenocosmia huwena . Evident differences were found in the HPLC chromatograms and MAIDI TOF spectrum of the venom from the two spiders. The amino acid sequences of the main neurotoxin from the two spiders are also different. The results indicated that, although the two spiders are similar to each other morphologically, they should belong to different species. The spider from Tongshi, Hainan was identified as a new species and was named Selenocosmia hainana sp. nov. Type specimens are deposited in College of Life Sciences, Hunan Normal University.

关 键 词：蜘蛛毒素 海南捕鸟蛛 新种 蛋白质 序列 

分 类 号：Q959.226.2[生物学—动物学]