压力超负荷大鼠心肌内分泌因子活化及相互作用  

ACTIVATION AND INTERACTION OF MYOCARDIAL ENDOCRINE FACTORS IN RATS STIMULATED BY PRESSURE OVERLOAD

作　　者：冯兵[1] 陈意生[2] 何作云[2] 黄敏[2] 周晓波[2] 

机构地区：[1]北京放射医学研究所 [2]第三军医大学新桥医院心内科,重庆400037

出　　处：《中国应用生理学杂志》1999年第4期329-331,共3页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金

摘　　要：目的：探讨压力超负荷是否诱导心肌内分泌活化及相互间的作用。方法：用放免法及比色法检测腹主动脉缩窄高血压大鼠心肌组织中血管紧张素Ⅱ、内皮素和一氧化氮含量的变化，并观察卡普托利对它们的作用。结果：腹主动脉缩窄大鼠动脉血压逐渐升高，术后４ ｈ 即显著升高；术后３０ ｍｉｎ 心肌组织中血管紧张素Ⅱ含量显著升高，此后并保持在高水平；心肌内皮素含量于术后２４ ｈ 开始并持续显著升高；心肌中一氧化氮含量却于术后１０ ｍｉｎ 迅速显著降低并持续受抑。小剂量卡普托利对大鼠血压无明显影响，但可完全抑制心肌中血管紧张素Ⅱ的升高，而且使内皮素活化滞后、一氧化氮含量降低减轻。结论：压力超负荷可诱导心肌血管紧张素Ⅱ、内皮素含量升高及一氧化氮含量降低，而心肌血管紧张素Ⅱ可加速内皮素活化。Aim: The purpose of the investigation was to determine whether the endocrine would be activated by pressure overload in rat induced by abdominal aorta constriction and the interaction between endocrine factors. Methods: By radioimmunoassay and colorimetry, we determined the changes of the content of myocardial angiotensin Ⅱ, endothelin and nitric oxide in rats after pressure overload and those after treated with captopril, then analyzed the relationship between the changes and pressure overload. Results: It was showed that the concentration of myocardial angiotensin Ⅱ increased significantly at 30 minutes after pressure overload, and then was kept in high level. The concentration of myocardial endothelin elevated markedly at 24h after operation, and was kept in high level since then. The elevation of Angiotensin Ⅱ was earlier than that of ET. Whereas, the concentration of myocardial nitric oxide decreased rapidly at 10 minutes after operation, and was kept in low level. The arterial pressure was not reduced and the effects of pressure overload, such as elevation of angiotensin Ⅱ was inhibited completely, rise of endothelin was delayed, and decrease of nitric oxide was alleviated by pretreatment with low dose of captopril. Conclusion: It was suggested that the elevation of myocardial angiotensin Ⅱ and endothelin and the decrease of myocardial nitric oxide could be induced by pressure overload, moreover the elevation of endothelin and decrease of nitric oxide could be accelerated and aggravated by angiotensin Ⅱ.

关 键 词：压力超负荷 血压紧张素 内皮素 心肌肥大 高血压 

分 类 号：R542.202[医药卫生—心血管疾病] R544.106[医药卫生—内科学]