反相高效液相色谱法同时测定多种头孢菌素的研究(I)  

Studies on the Simultaneous Measurement of Several Cephalosporins by RP-HPLC (I)

作　　者：吴振洁[1] 郭伟斌[1] 张启国[1] 倪坤仪[2] 林永顺[1] 

机构地区：[1]厦门市药品检验所,福建厦门361012 [2]中国药科大学分析化学教研室,江苏南京210009

出　　处：《色谱》1999年第6期518-521,共4页Chinese Journal of Chromatography

基　　金：福建省卫生厅科研经费资助

摘　　要：建立了同时分离测定６种头孢菌素（头孢米诺、头孢羟氨苄、头孢克罗、头孢氨苄、头孢拉定和头孢西丁）的高效液相色谱法。流动相为Ｖ（５０ ｍ ｍ ｏｌ／Ｌ的磷酸二氢钾缓冲液，ｐＨ ３．４）ｖＶ（乙腈）＝ ８７．５ｖ１２．５的混合液，色谱柱为ＨｙｐｅｒｓｉｌＯＤＳＣ１８５ μｍ ，２００ ｍ ｍ ×４．６ ｍ ｍ ｉ．ｄ．，紫外检测波长为２５４ ｎｍ ， 流速为１．０ ｍ Ｌ／ｍ ｉｎ。各个组分的线性范围：头孢米诺为１６４ ｎｇ～１６．４ μｇ，头孢羟氨苄为９９ ｎｇ～９．９３４ μｇ，头孢克罗为１０４ ｎｇ～１０．３５８ μｇ，头孢氨苄为１２２ ｎｇ～１２．２２４ μｇ，头孢拉定为１０７ ｎｇ～１０．７０２ μｇ，头孢西丁为１１５ ｎｇ～１１．５０６ μｇ。各组分的方法回收率及相对标准偏差：头孢米诺为（１０３．５３±１．２）％ ，头孢羟氨苄为（９９．３５±０．７）％ ，头孢克罗为（１０１．３９±０．７）％ ，头孢氨苄为（１０１．４５±１．８）％ ，头孢拉定为（９８．６８±１．９）％ ，头孢西丁为（９７．５９±１．４）％ 。This paper reported the research on the simultaneous separation and determination of six cephalosporins by RP\|HPLC. Six cephalosporins are cefalcor ,cefalexin, cefradine, cefadroxil, cefominox and cefoxitin .The analytical conditions for this method were as follows: a Hypersil ODS C 18 (200 mm×4.6 mm i.d., 5 μm), detection wavelength: 254 nm; a mobile phase solution of 50 mmol/L monopotassium phosphate (pH 3.4)\|acetonitrile(87.5∶12.5) and DAD detector. The flow rate was 1.0 mL/min. The calibration curves of the six compounds were linear, the correlation coefficients were 0.995 1 for cefominox, 0.999 9 for the others ,the range were 164 ng\|16.4 μg for cefominox, 99 ng\|9.934 μg for cefadroxil, 104 ng\|10.358 μg for cefalcor, 122 ng\|12.224 μg for cefalexin, 107 ng\|10.702 μg for cefradine and 115 ng\|11.506 μg for cefoxitin. The recovery rates were 103.5% for cefominox,99.3% for cefadroxil,101.4% for cefalcor,101.5% for cefalexin,98.7% for cefradine and 97.6% for cefoxitin . Six cephalosporins were all stable in 50 mmol/L monopotassium phosphate (pH 3 4\|4 6).When preparations of these cephalosporins were determined, it is indicated there were no difference between the results by using this method and the pharmacopoeia methods .The total separation time of these cephalosporins was within fifteen minutes. This method is simple, sensitive, rapid and accurate.

关 键 词：内酰胺抗生素 头孢菌素 头孢拉定 头孢氨苄 测定 

分 类 号：R978.11[医药卫生—药品]