毛细管区带电泳法分析头孢菌素类药物的纯度  

Purity Analysis of Cephalosporins with Capillary Zone Electrophoresis

作　　者：龙虹[1] 丁强[1] 王天松 黄爱今[1] 孙亦梁[1] 

机构地区：[1]北京大学化学学院,北京100871

出　　处：《色谱》1999年第6期570-572,共3页Chinese Journal of Chromatography

基　　金：教育部博士点基金

摘　　要：提出了以ｐＨ ９．２０的２０ ｍ ｍ ｏｌ／Ｌ硼酸缓冲溶液、ｐＨ ６．８６的２０ ｍ ｍ ｏｌ／Ｌ磷酸缓冲溶液和ｐＨ ２．０５ 的５０ｍ ｍ ｏｌ／Ｌ磷酸缓冲溶液作为背景电解质分析９种头孢菌素类抗生素药物纯度的毛细管区带电泳方法。用内部归一化法定量，方法简单快速。讨论了方法的优点和局限性。A capillary zone electrophoresis method is proposed for the purity determination of nine cephalosporin drugs. A background electrolyte comprising of either 20mmol/L pH 9.20 borate buffer or 20mmol/L pH 6.86 phosphate buffer was used for most drugs studied except for cefaloridine which formed neutral molecules at these pH values. For it 50 mmol/L pH 2.05 phosphate buffer was used instead. Internal normalization method was employed for quantitation. The method is simple, rapid and versatile. Analysis was completed within 8min. The merits and limitations of the method were also discussed.

关 键 词：毛细管区带电泳 纯度 测定 头孢菌素 

分 类 号：R978.11[医药卫生—药品]