高效薄层扫描法测定蛛网膜下腔出血患者脑脊液中血小板活化因子  

High Performance Thin Layer Chromatographic (HPTLC) Determination of PAF in Cerebrospinal Fluid of Patients with Subarachnoid Hemorrhage(SAH)

作　　者：王荣 张新江[2] 胡晓丽 赵兴红 

机构地区：[1]兰州军区后勤部药物研究所,甘肃兰州730020 [2]兰州军区总医院,甘肃兰州730000 [3]兰州军区医学高等专科学校,甘肃兰州730020

出　　处：《色谱》1999年第6期590-592,共3页Chinese Journal of Chromatography

摘　　要：建立了测定蛛网膜下腔出血患者（ＳＡＨ）脑脊液中血小板活化因子（ＰＡＦ）质量浓度的高效薄层扫描方法。薄层板为高效硅胶Ｇ板（１００ ｍ ｍ ×１００ ｍ ｍ ），展开剂为Ｖ（氯仿）ｖＶ（甲醇）ｖＶ（水）＝ ６５ｖ３５ｖ６，下层液展开，显色剂为１００ ｇ／Ｌ磷钼酸溶液，展开距９０ ｍ ｍ ，扫描波长６３０ ｎｍ 。方法的线性范围０．５ ～２．５ μｇ／Ｌ，相关系数０．９９９ ０，最小检测质量浓度５０ ｎｇ／Ｌ，平均回收率９８．６％ 。运用所建立的方法测定了１６例ＳＡＨ患者发病后脑脊液和１０例非神经系统疾病患者脑脊液中ＰＡＦ的质量浓度及其变化规律，为临床提供了诊断依据。The Platelet Activating Factor (PAF) is believed to be the major function in human after cerebral vascular spasm and cerebral ischemia. PAF has been found to participate in cerebral vascular spasm and cerebral ischemia by the basic and clinical study. The symptom of cerebral vascular spasm and cerebral ischemia has appeared with SAH. It has not been reported that the rule and change of PAF with SAH. In the present work, the concentration of PAF in human cerebral spinal fluid (CSF) with SAH were determined by high performance layer thin chromatography. The TLC plate was coated with high performance silica gel G using V (chloroform)∶ V (methanol)∶ V (water)=65∶35∶6 as developing solvent. The PAF was detemined by TLC scanning method and detected at 630nm. The method was applied to determine the concentration of PAF in 16 CSF samples with SAH. The samples were collected in 1\|3 d, 7\|10 d and 14\|21 d.CSF samples were deproteinized with methanol and chloroform. After centrifugation, the chloroform layer separated was dried at room temperature with nitrogen and stored under 20 ℃ in the refrigerator. The linear range of the method was 0.5\|2.5 μg/L with regression coefficient of 0.999 0 .The lower limit of detection were 50 ng/L. The recovery of the method was 98.6%.The method enables a simple, rapid and reproducible quantification of PAF with SAH.

关 键 词：血小板活化因子 蛛网膜出血患者 脑脊液 测定 

分 类 号：R446.112[医药卫生—诊断学]