陆川猪耳部成纤维细胞的体外培养  

In vitro culture of ear fibroblast cells from Lu-chuan pig

作　　者：秦小娥[1] 胡林林[1] 卢晟盛[1] 卢克焕[1] 

机构地区：[1]广西大学动物繁殖研究所广西亚热带生物资源保护利用重点实验室,广西南宁530004

出　　处：《中国兽医学报》2011年第8期1189-1191,1195,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30860039);农业部"转基因生物新品种培育重大专项"项目(2009ZX08010-023B);广西科学研究与技术开发计划资助项目(桂科能0815011-6-3)

摘　　要：本研究建立陆川猪耳部成纤维细胞的体外培养体系,采用组织块培养法可以获得陆川猪耳部成纤维细胞。用0.25%胰蛋白酶+0.02%EDTA消化液消化细胞、用含有10%FBS的DMEM对细胞进行培养,能很好的支持陆川猪耳部成纤维细胞的生长。传3代后,观察到培养的细胞形态逐渐均一,为典型的成纤维细胞,绝大部分呈梭形或不规则三角形。细胞生长时呈放射状、火焰状或旋涡状走势。用间接免疫荧光法对细胞类型进行鉴定。抗波形蛋白免疫荧光染色显示为阳性,抗角形蛋白免疫荧光染色为阴性。结果,分离得到的细胞为成纤维细胞。结果表明,用组织块培养法,可以培养出陆川猪耳部成纤维细胞。In order to establish an isolation method and culture system for Lu-chuan pig ear fibroblast cells,Lu-chuan pig ear fibroblast cell was isolated and cultured by tissue-piece methods.Using 0.25% trypsin ＋ 0.02% EDTA as digested medium and DMEM containing 10% FBS as culture medium for subculture,it was concluded that culture system was good enough for lu-chuan pig ear fibroblast cells.The pured fibroblast cells could be obtained after passing 3 subcultures.The cultured cells were typical fibroblast morphology,which grooved as irregular triangle or shuttle,appearing as radioactive blaze and helix in shape.The cell type was identified by fluorescent immuneocytochemistry（ICC） and the result indicated that isolated cells from Lu-chuan ear tissues were Lu-chuan ear fibroblast cells.In conclusion,Lu-chuan pig ear fibroblast cell could be isolated and cultured by tissue-piece methods.

关 键 词：陆川猪 耳部成纤维细胞 组织块培养 间接免疫荧光 

分 类 号：Q813[生物学—生物工程]