不同激活方法对小鼠卵母细胞孤雌发育及二倍体形成的影响  

Effects of different activation methods on parthenogenetic development and diploid formation of mouse oocytes

作　　者：赵天闯[1] 张鹏[1] 陈玥[1] 高飞[1] 唐博[1] 李子义[1] 

机构地区：[1]吉林大学畜牧兽医学院动物胚胎工程吉林省重点实验室,吉林长春130062

出　　处：《中国兽医学报》2011年第8期1218-1222,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30901056);博士点新教师基金资助项目(20090061120010)

摘　　要：卵母细胞的孤雌激活是研究哺乳动物受精机制和发育机理的有效方法,也是细胞核移植、显微注射受精技术、孤雌胚胎干细胞等研究内容中的重要环节。本试验分别用乙醇、SrCl2、钙离子载体A23187对小鼠卵母细胞进行单独孤雌激活,并分别与6-DMAP联合运用,对小鼠卵母细胞进行联合孤雌激活。结果显示:(1)不同激活剂单独或联合激活,对卵母细胞的激活率有显著影响(P<0.05),SrCl2组的激活率最高(92%～94%);(2)相同激活剂对卵母细胞孤雌囊胚的发育率在单独激活组(SrCl2组,18%)和联合激活组(SrCl2+6-DMA组,53%)中存在显著差异(P<0.05);(3)相同激活剂对卵母细胞二倍体率在单独激活组(钙离子载体A23187组,21%)和联合激活组(钙离子载体A23187+6-DMA组,77%)中均存在显著差异(P<0.05)。结果表明:(1)SrCl2可以对小鼠卵母细胞进行有效的孤雌激活;(2)联合激活法可以显著提高孤雌卵母细胞的囊胚发育率;3、6-DMAP可以抑制第二极体排出,显著提高孤雌激活卵母细胞的二倍体率。Parthenogenetic oocyte activation is an effective method for studies of mechanisms of mammalian fertilization and development,and an important part for studies of nuclear transfer,microinjection fertilization techniques,parthenogenetic embryonic stem cell research as well.Effects of ethanol,SrCl2 and Ca-A23187 on parthenogenetic activation rate and diploid rate of mouse oocytes,either individually or in combination with the use of 6-DMAP were investigated in this study.Our results showed：activation rates of mouse oocytes treated with either single or joint activators presented significant differences（P0.05）.Activation rate of oocytes treated with SrCl2 was the highest（92%-94%）;blastocyst rates of oocytes treated with the SrCl2 alone or combination with 6-DMAP were significant differences（P0.05） between single group（18%） and that（53%） in joint group.3.There were significant differences（P0.05） of diploid rates between single group（21%） and joint group（77%） when oocytes were treated with Ca-A23187 or combined with 6-DMAP.Our results demonstrate：the SrCl2 has significant effects on parthenogenetical activation of mouse oocytes;the parthenogenetical blastocyst rates are significantly improved in the joint groups with 6-DMAP;the 6-DMAP can inhibit the extrusion of second polar body,and significantly increase diploid rate of parthenogenetic activation mouse oocytes.

关 键 词：孤雌激活 二倍体 小鼠 

分 类 号：S814[农业科学—畜牧学]