2,4-D浓度对玉米花粉诱导小麦产生单倍体幼胚得胚率的影响  

Effect of 2,4-D Concetration on Maize Pollen Induced Immature Wheat Haploid Embryos Formation

作　　者：蔡东明[1] 吉万全[1] 王长有[1] 张宏[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100

出　　处：《种子》2011年第7期56-58,共3页Seed

基　　金：陕西省自然科学基金项目"杂交小麦新方法的研究及利用(2008ISM06)"

摘　　要：在玉米花粉诱导普通小麦产生单倍体技术中,2,4-D浓度是影响幼胚得胚率的重要因素之一。为了确定2,4-D的适宜浓度,利用9个玉米材料和16个小麦材料进行了研究。在小麦散粉前和散粉后,授玉米花粉后12 h和36 h分别用150、200、250、300、350 mg/L的2,4-D蘸穗2次,调查数据显示,散粉前处理得胚率平均值分别为6.74%、7.19%、8.44%、7.53%和6.61%;散粉后分别为2.53%、3.11%、4.70%、2.81%和2.67%。结果表明,小麦散粉前授玉米花粉后用250 mg/L的2,4-D处理得胚率最高。2,4-D concetration is one of the important factors during maize pollen inducing immature wheat haploid embryo formation.In order to obtain the optimal 2,4-D concetration,9 maize materials and 16 wheat materials were studied.Wheat spike was pollinated with maize pollen before and after disseminating pollen,thenthe spike were treated with 150,200,250,300 and 350 mg/L 2,4-D 12 h and 36 h.The date showed that embryo formation frequency were 6.74%,7.19%,8.44%,7.53% and 6.61% when treated before disseminating pollen and 2.53%,3.11%,4.70%,2.81% and 2.67% after disseminating pollen,respectively.The results desmontrated that the frequency of embryo formation was the highest when spikes was treated with 250mg/L 2,4-D before disseminating pollen.

关 键 词：玉米 小麦 得胚率 

分 类 号：S512.1[农业科学—作物学]