正交实验法优化转基因玉米中植酸酶的提取工艺  

Optimization of the Extract Technology on the Phytase from Transgenic Corn by Orthogonal Experiment

作　　者：刘娜[1] 柳鸿敏[2] 于壮[1] 张欣芳[1] 盖春宇[2] 邢珍娟[1] 陈奕冰[2] 李静[2] 张明[1] 

机构地区：[1]吉林省农业科学院生物技术研究中心,长春130033 [2]吉林大学公共卫生学院,长春130021

出　　处：《玉米科学》2011年第4期142-144,148,共4页Journal of Maize Sciences

基　　金：转基因生物新品种培育重大专项课题(2008ZX08012-04;2008ZX08011-003)

摘　　要：采用正交实验法研究pH值、料液比和提取时间等因素对酶提取效率的影响,探索超声提取转基因玉米中植酸酶最佳提取条件。结果显示,最佳提取条件为提取液pH值5.5,料液比为1∶4,提取时为15 min。Orthogonal test method was used to study the ultrasonic extraction conditions of the phytase from transgenic corn. It was found that the content of the phytase extracted was the highest under the conditions of ultrasonic extraction for 15min, using pH 5.50 buffer liquid at a liquid-solid ratio of 4 tol, which guaranteed for the evaluation of the transgenic corn.

关 键 词：转基因玉米 植酸酶 正交实验 

分 类 号：S513[农业科学—作物学]