兔肺VX2肿瘤手术切除模型中外周血CD4^＋CD25^＋调节性T细胞的变化和意义  

The change and significance of CD4^＋CD25^＋ regulatory T cells in peripheral blood in rabbit＇ s lung VX2 tumor resection model

作　　者：陈少慕[1] 黄海涛[1] 何靖康[1] 李忠诚[1] 马海涛[1] 

机构地区：[1]苏州大学附属第一医院心胸外科,215006

出　　处：《中华实验外科杂志》2011年第8期1238-1240,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察外周血CD4^＋CD25^＋调节性T细胞水平在兔肺VX2肿瘤手术切除模型中的变化。方法将30只纯种新西兰白兔随机分成3组。A组：建立兔肺VX2肿瘤手术切除模型；B组：建立兔肺VX2肿瘤模型，不进行肿瘤切除术；C组：不建立兔肺VX2肿瘤模型，仅行兔肺叶切除术。3组实验兔在相同的时间点抽取外周血，经流式细胞仪分析测定外周血CD4^＋CD25^＋调节性T细胞的水平。结果兔在种植肿瘤后外周血CD4^＋CD25^＋调节性T细胞的水平增高（P〈0．01）；手术切除兔肺VX2肿瘤后15d和30d，实验兔外周血CD4^＋CD25^＋调节性T细胞水平较术前下降（P〈0．05），而不进行肿瘤切除的实验兔外周血CD4^＋CD25^＋调节性T细胞水平持续增高（P〈0．05）。结论外周血CD4^＋CD25^＋调节性T细胞水平升高可能与肿瘤的发生发展相关；手术切除肿瘤可减少肿瘤负荷，有利于机体抗肿瘤免疫功能的恢复。Objective To analyze the change of CD4^＋CD25^＋ regulatory T cells in peripheral blood in rabbit＇ s lung VX2 tumor resection model. Methods Thirty New Zealand rabbits were divided into 3 groups： group A, 10 rabbits with lung VX2 tumor receiving lobeetomy; group B, 10 rabbits with lung VX2 tumor receiving no operation; group C, 10 rabbits without lung VX2 tumor receiving lobeetomy only. The peripheral blood of rabbits was drawn. All the samples were analyzed by flow cytometry （FCM）. Results The proportion of the CD4^＋CD25^＋ regulatory T cells in peripheral blood of the rabbits was increased after the implantion of VX2 tumor （P 〈0. 01 ）, that in the rabbits with VX2 lung tumor receiving tumorectomy was obviously decreased at the 15th and 30th day after the operation （P 〈0. 05）, but that in the rabbits with VX2 lung tumor receiving no operation did not have the same change （ P 〈 0. 05 ）. Conclusion The increase of the proportion of CD4^＋CD25^＋ regulatory T cells in peripheral blood is related to immunosuppression. The functional disorder of immune system recovers in a certain extent after resection of the tumor.

关 键 词：肺肿瘤 调节性T细胞 手术 流式细胞仪 

分 类 号：R392[医药卫生—免疫学]