免疫球蛋白结合蛋白在肝癌中的表达及其意义  

The expression and significance of the correlation factor of unfolded protein response： Bip in hepatoma

作　　者：吕海涛[1] 刘三光[1] 穆宏凌[2] 边伟[1] 刘建华[1] 刘润田[1] 高翔[3] 

机构地区：[1]河北医科大学第二医院肝胆外科,石家庄050000 [2]河北省卫生厅考试培训中心 [3]河北医科大学第二医院血管外科

出　　处：《中华实验外科杂志》2011年第8期1312-1314,共3页Chinese Journal of Experimental Surgery

摘　　要：目的检测免疫球蛋白结合蛋白（Bip）在肝癌组织中的表达，探讨肝癌发生发展过程中存在未折叠蛋白反应（UPR）的激活。方法采用逆转录一聚合酶链反应（RT—PCR）检测新鲜的42例肝癌标本、15例同个体癌旁1．0cm肝组织和15例正常肝组织中Bip的mRNA表达，应用Westernblot方法验证57例肝癌组织和正常肝组织标本中Bip在蛋白水平的表达。结果肝癌组织、癌旁肝组织、正常肝组织中BipmRNA相对表达量分别为0．4772±0．0401、0．4332±0．0488、0．4301±0．0398，BipmRNA在3组之间的表达呈下降趋势（P〈0．05）；在肝癌组织和正常肝组织中均有Bip蛋白的表达，但肝癌组织中的表达明显高于正常肝组织。结论在肝癌的生长过程中存在未折叠蛋白反应的激活。肝癌组织中BipmRNA和蛋白的表达一致，均高表达。在肝癌的发生过程中，未折叠蛋白反应具有蕈要作用。Objective To detect the express of immunoglobulin-binding protein （Bip） mRNA in the tissue of hepatoma;demonstrating that it exist activation of unfold protein response （UPR） in process of growth, and the expression of UPR was higher than normal tissue. Methods To study the mRNA of Bip in 42 case example of hepatome, 15 case paired adjacent hepatic tissues to carcinoma and 15 case normal hepatic tissues by RT-PCR. To qualitation check the expression of Bip in the level of protein by Western blotting. Results The express of Bip mRNA in hepatoma tissue,adjacent hepatic tissue to carcinoma and normal hepatic tissue respectively was 0. 4772 ± 0. 0401,0. 4332 ± 0. 0488,0. 4301 ± 0. 0398 ; the express of Bip mRNA was higher in hepatoma than others （ P 〈 0. 05 ）. The experiment indicate ： the Bip express in the hepatoma tissue and normal hepatic tissue, but it was higher in hepatoma tissue than in the normal hepatic tissue. Conclusion It exist activation of UPR in process of growth;The express of Bip protern and its mRNA was accordant, it was higher in hepatoma tissue than in normal hepatic tissue. The changes of its gene and protern is possible to have the relationship with the carcinogenesis, growth and transfer of hepatoma. And it may have significant effect in molecule mechanism of hepatoma. It also descripte that unfold protein response may have very important role in the pocess of carcinogenesis.

关 键 词：肝癌 肝组织 免疫球蛋白结合蛋白 RT—PCR WESTERN BLOT 

分 类 号：R711.75[医药卫生—妇产科学]