前列地尔对兔肾缺血再灌注时细胞凋亡的保护作用  

Alprostadil effects of prostavasin on apoptosis by renal ischemia-reperfusion injury in rabbits

作　　者：郭凌燕[1] 胡世雄[2] 

机构地区：[1]广州军区广州总医院157附属医院药械科,510510 [2]广东省人民医院血管甲状腺腹壁疝外科

出　　处：《中华实验外科杂志》2011年第8期1356-1358,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察前列地尔对兔肾缺血再灌注损伤时肾小管上皮细胞凋亡的保护作用。方法建立兔肾缺血再灌注损伤动物模型，将实验兔随机分为3组：即对照组、缺血再灌注组和前列地尔组，每组10只。检测兔血清肌苷（cr）、尿素氮（BUN）浓度及肾组织中丙二醛（MDA）、超氧化物歧化酶（SOD）和髓过氧化物酶（MPO）含量及肾组织中凋亡细胞。结果与对照组比较，缺血再灌注组和前列地尔组在再灌注后Cr、BUN水平均大幅度上升（P〈0．05）；但前列地尔组动物在再灌注60min后Cr水平（231．32±17．57）μmol／L明显低于缺血再灌注组（390．61±20．42）μmol／L（P〈0．05）；肾小管上皮细胞bcl-2、bax、Caspase-3表达与对照组比较，缺血再灌注组明显增强（P〈0．05）；前列地尔组与缺血再灌注组比较表达减弱，但仍强于对照组（P〈0．05）。前列地尔组、缺血再灌注组与对照组比较凋亡细胞数增多，前列地尔组与缺血再灌注组比较凋亡细胞数减少。MDA、SOD与MPO的活性与对照组比较，缺血再灌注组与前列地尔组明显增强（P〈0．05）；前列地尔组与缺血再灌注组比较，该两者活性明显减弱（P〈0．05）。结论前列地尔在肾脏缺血再灌注损伤时能有效的保护肾功能其作用机制可能是通过减少细胞脂质过氧化，从而降低bcl-2、bax、Caspase-3等凋亡基因的表达。Objective To study the alprostadil effects of alprostadil on apoptosis by renal ischemi- a-reperfusion injury （IRI） in rabbits. Methods The rabbit IRI models were made, and randourly divided into three groups： control group, IRI group and prostavasin intervention group. The creatinine （Cr） and blood urea nitrogen （BUN） were determined. Malondialdehyde （MDA）, superoxide dismutase （SOD） , myeloperoxidase （MPO）, bcl-2, bax, Caspase-3 and apoptosis were assayed at 60 min after reperfusion. Results The Cr and BUN levels in plasma in IRI group and Prostavasin intervention group were increased obviously after reperfusion. The Cr levels at 60 min after reperfusion in alprostadil intervention group （231.32± 17.57） μmol/L were significantly lower than in IRI group （390. 61 ±20.42） μmol/L, （P 〈 0. 05）. The levels of bcl-2, bax, Caspase-3 in the renal tissue in IRI group were significantly higher than in control group （ P 〈 0. 05 ） , and those in alprostadil intervention group were lower than in IRI group, but markedly higher than in control group （ P 〈 0. 05 ）. The number of apoptotic cells in alprostadil intervention group and IRI group was increased as compared with control group, and that in alprostadil intervention group was reduced as compared with IRI group. The contents of MDA, SOD and MPO in renal tissue of IRI group and Prostavasin intervention group were significantly higher than in control group （ P 〈 0. 05 ） , and those in IRI group were significantly lower than in aiprostadil intervention group （ P 〈 0.05 ）. Conclusion Alprostadil could be used to protect renal ischemia-reperfusion injury probably by decreasing oxygen free radicals generation, inhibiting neutrophils aggregating and activating in the renal tissues, thereby inhibiting the expression of bcl-2, bax, Caspase-3.

关 键 词：前列地尔 肾缺血 再灌注损伤 脱噬作用 

分 类 号：R285.5[医药卫生—中药学]