白木纳格葡萄败育型胚珠培养研究  

A Study on Technique for Vestigal ovules Culture of White Munake Grape

作　　者：夏培蓓[1] 伍新宇[2] 吴玉霞[3] 朱瑜[1] 陈新红 阿尔孜古丽.卡斯木 罗淑萍[1] 

机构地区：[1]新疆农业大学农业生物技术重点实验室,乌鲁木齐830052 [2]新疆农业科学院园艺作物研究所,乌鲁木齐830091 [3]新疆农业大学林学与园艺学院,乌鲁木齐830052 [4]新疆克孜勒苏柯尔克孜自治州农业技术推广中心,新疆阿图什845350

出　　处：《新疆农业科学》2011年第7期1201-1204,共4页Xinjiang Agricultural Sciences

基　　金：国家"十一五"科技支撑计划项目(2007BAD36B01-3);新疆维吾尔自治区高技术研究发展计划项目(200911110);新疆维吾尔自治区重大科技专项项目(200731136);新疆维吾尔自治区果树学重点学科基金

摘　　要：【目的】研究白木纳格葡萄败育型胚珠离体培养的最优方案,为木纳格葡萄胚挽救育种技术的研究奠定基础。【方法】以花后不同天数的白木纳格葡萄败育型胚珠为试材,进行离体培养,研究接种时间、基本培养基种类I、BA浓度以及活性炭对胚珠发育率的影响。【结果】白木纳格葡萄败育型胚珠取样适宜期为DAF35至DAF40,离体培养后,胚珠发育率均可达到50%以上,其中DAF 40 d为最佳取样时间,胚珠发育率可达到61.07%。在IBA 1.5 mg/L,GA30.5 mg/L,蔗糖60 g/L,琼脂5 g/L,活性炭1 g/L的Nitsch培养基中,白木纳格葡萄败育型胚珠发育率最高。【结论】建立了适合白木纳格葡萄败育型胚珠培养体系。【Objective】The purpose of this program was to research the optimal design of self-pollination of white Munake grape vestigial ovule that was used as test materials in this experiment,which would lay a theoretical basis of embryo rescue.【Method】The berries of white Munake grape were collected in different days after flower drop.The vestigal ovules were inoculated on medium used with various composition,and they were studied at the different sampling times.And the different basic medium,different concentration IBA and different concentration AC of vestigal ovules on the effect of culture were studied,too.【Result】The results showed that the best inoculation period of white Munake grape vestigal ovules was 35-40 days after flower drop stage,and in Nitsch medium supplemented IBA 1.5 mg/L,GA3 0.5 mg/L,suger 60 g/L,TC agar 5 g/L and AC 1 g/L,the percentage of ovules developed the highest.【Conclusion】This study provided the culture system of the vestigal ovule from white Munake grape.

关 键 词：白木纳格葡萄 败育型胚珠 胚珠培养 

分 类 号：S663.1[农业科学—果树学]