金匮肾气丸含药血清对体外兔BMSCs增殖细胞周期及凋亡的影响  

Effect of serum containing Jingui Shenqi pill on proliferation,cellcycle and apoptosis of rabit BMSCs in vitro

作　　者：张文信[1] 刘金文[1] 曹学伟[1] 叶青合[1] 

机构地区：[1]广州中医药大学第二临床医学院,广东广州510120

出　　处：《辽宁中医杂志》2011年第8期1511-1514,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：中国博士后科学基金(20100470899)

摘　　要：目的:研究金匮肾气丸含药血清对体外兔骨髓间充质干细胞(bone marrow mesenchymal stem cells BMSCs)增殖,细胞周期及凋亡的影响。方法:使用全骨髓贴壁法分离兔BMSCs,通过MTT法检测金匮肾气丸含药血清对BMSCs活性的影响,通过流式细胞仪(Fow Cytometry FCM)检测金匮肾气丸含药血清对地塞米松(dexamethasone DEX)诱导的BMSCs的凋亡率和细胞周期的影响。结果:金匮肾气丸各组光吸收值(OD)高于空白对照组。金匮肾气丸各组与模型组比较,Go/G1期细胞比例降低,S期和G2/M期细胞比例升高,凋亡率降低,并呈一定的量效依赖关系。结论:金匮肾气丸含药血清体外能够提高兔BMSCs的活性,促进G0/G1期的BMSCs向S期和G2/M期转化,促进BMSCs的增殖,降低地塞米松所致的兔BMSCs的凋亡率。Objective：To study the effect of serum containing Jingui Shenqi pill on proliferation , cell cycle and apoptosis of rabit bone marrow mesenchymal stem cells（BMSCs） in vitro. Methods ：Rabit BMSCs were isolated by the whole bone marrow adherence method. The effect of serum containing Jingui Shenqi pill on the proliferation of rabit BMSCs was detected by MTT, the effect of serum containing Jingui Shenqi pill on cellcycle and apoptosis rate of rabit BMSCs induced by dexamethasone （DEX） was measured by Fow Cytometry （FCM）. Results ： The light absorption of Jingui Shenq was higher than blank control. Compared with model group,the percentage of rabit BMSCs inall the Jingui Shenqi pill groups was decreased at the G0/G1 phase and was in- creased at the S phase and G2/M phase in a dose -dependent manner,and the apoptosis of rabit BMSCs was decreased in a dose - dependent manner. Conclusion ： The serum containing Jingui Shenqi pill can increase the activity of rabbit BMSCs in vitro , pro- moted transformation of the BMSCs at the G0/G1 phase to the S phase and Ga/M phase,promoted proliferation of the BMSCs ,decreased the apoptosis rate of rabit BMSCs induced by DEX.

关 键 词：金匮肾气丸 骨髓间充质干细胞 增殖 细胞周期 凋亡 

分 类 号：R2-03[医药卫生—中医学]