揉髌手法对兔膝关节软骨细胞凋亡及超微结构的影响  

The Effects Of The Rou-Bin Manipulation To The Apoptosis And Ultrastructure Of Cartilage Cell With Rabbits's Knee Joints

作　　者：戴七一[1] 文宗振[2] 袁经阳[1] 林光琪[3] 刘靖[3] 阮萍[1] 黎强[3] 覃学流[1] 滕居赞[1] 容向宾[1] 韩杰[1] 覃杰[1] 王雄[1] 吴兆沛[1] 

机构地区：[1]广西中医学院附属瑞康医院,广西南宁530011 [2]广西大学,广西南宁530004 [3]广西中医学院,广西南宁530001

出　　处：《辽宁中医杂志》2011年第8期1662-1665,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30860359);广西科技厅自然基金资助项目(桂科自0832178)

摘　　要：目的:探讨揉髌手法对兔膝关节软骨细胞凋亡及超微结构的影响,进而探讨揉髌手法治疗膝关节骨性关节炎的可能作用机制。方法:采用50只新西兰兔,随机分为手法组、针剂组、模型对照组、假模组和正常组各10只。前3组通过手术结扎并切断右侧臀下静脉、股静脉和大隐静脉造模,假模组显露相应血管但不结扎,正常组不做任何处理。造模1周后手法组采用揉髌手法分别施术于兔右下肢5周共17次;针剂组右膝关节内每周注射一次玻璃酸钠液,连用5周。其余3组不做任何处理。造模后8周末处死所有动物,取右膝关节内侧胫骨平台软骨组织,采用原位末端标记法检测软骨细胞凋亡率,透射电镜观察软骨组织超微结构变化,数据经统计学处理。结果:模型组软骨组织退变明显,细胞凋亡率(17.25±3.24)%,电镜提示凋亡细胞较多;假模组软骨组织退变明显,细胞凋亡率(16.63±2.13)%,电镜提示细胞结构保存较差;手法组、针剂组软骨组织退变较轻,手法组细胞凋亡率(8.40±2.84)%,电镜提示细胞结构保存较好;针剂组细胞凋亡率(9.50±3.25)%,电镜提示细胞结构保存尚可;正常组软骨组织正常。细胞凋亡率(3.88±1.23)%,电镜提示细胞结构基本正常。与模型组比较,手法组、针剂组和正常组细胞凋亡率均P<0.01,有显著性差别。结论:揉髌手法可降低兔膝关节软骨细胞凋亡率,进而维护软骨组织的正常结构。Objective：To explore the effect of the Rou- Bin manipulation to the apoptosis and uhrastructure of cartilage cell with rabbit＇ s knee joints, and the mechanism of Rou - Bin manipulation to the knee joint cartilage tissue of the rabbits. Methods ： 50 rabbits were randomly divided into manipulation group, injection group, model group, dummy - model group and normal group, 10 for each group, ligaturing and cutting off the inferior - gluteal vein, femoral vein and great saphenous vein on the right side of the rabbit for the first 3 groups, shows the same veins without cutting off for dummy - model group, no treatment fur the normal group. 1 week after modeled, 17 times of Rou -Bin manipulation on each rabbit in manipulation group, the rabbit injection with sodium hyaluronate 5 times in inject group, no special treatment for other rabbits. The 8th weekend after modeled, to take all the rabbit right knee medial tibial plateau cartilage tissue processing, detected chondrocyte apoptosis rate by TUNEL, to observe the change of the ultrastructure of cartilage cell by TEM, statistics processing. Results ： Cartilage degeneration clearly for the model group, chondrocyte apoptosis rate is 17.25 ±3.24%, hint by TEM ：many apoptosis chondrocyte; Cartilage degeneration clearly for dummy -model group,chondrocyte apoptosis rate is 16. 63± 2. 13% ;Cartilage degeneration lightly for the manipulation group and inject group,chondrocyte apoptosis rate is 8.40 ± 2. 84% for manipulation group, hint by TEM：good structure with the cell;chon- drocyte apoptosis rate is 9.50 ± 3.25 % for inject group, hint by TEM：good structure with the cell; no cartilage degeneration for the normal group, chondrocyte apoptosis rate is 3. 88 ±1.23%, hint by TEM：the structure with the cell nearly normal. To compare with model group, all proportions of the chondrocyte apoptosis rate for the manipulation group,inject group and normal group are 〈0. 01, statistically significant difference. Conclusion：The Rou - Bin manipulation can help the

关 键 词：手法 兔 膝关节 细胞凋亡 透射电镜 

分 类 号：R244.1[医药卫生—针灸推拿学]