稻瘟病菌无毒基因Avr-Pid2的分子标记及其连锁图  

Molecular Markers and the Linkage Map of Avr-Pid2 in the Rice Blast Fungus Magnaporthe oryzae

作　　者：翟焕趁[1] 王宝华[2] 鲁国东[2] 朱立煌[3] 王宗华[1,2] 

机构地区：[1]福建农林大学生命科学学院,福建福州350002 [2]福建农林大学植物保护学院,福建福州350002 [3]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《热带作物学报》2011年第6期1136-1143,共8页Chinese Journal of Tropical Crops

基　　金：国家公益性行业计划(200803008);国家自然科学基金(30400260;3000111)

摘　　要：无毒基因的克隆与变异监测可以为水稻抗病品种布局与利用提供重要信息。将菌株GUY11和FJ81278及其有性后代接种水稻品种Pi-d2及其亲本TP309进行毒性分析。结果表明,FJ81278含Avr-Pid2,有性后代在Pi-d2上的无毒、有毒分离比例符合1:1,推断FJ81278对Pi-d2的无毒性由单一基因座控制。通过SSR标记和转座子元件标记分析,确定Avr-Pid2定位于染色体7上,并获得了与无毒基因Avr-Pid2连锁的标记Propiz-t和ms7-27/28,它们与Avr-Pid2的遗传距离分别为6.1 cM、13.0 cM。这些标记的获得将Avr-Pid2限制在第7染色体上约420 kb物理距离范围内,为进一步的克隆奠定了良好基础。Cloning and variation of avirulenee genes will provide important information to support rice resistance gene deployment. The progeny isolates of FJ81278 and GUY11 were inoculated on a transgenie line with Pi-d2 and its parent variety TP309 to assay their virulence. Results showed that the isolate FJ81278 had the Avr-Pid2 gene, and the segregation ratio of avirulenee and virulence on Pi-d2 fit 1：1, suggesting it was a single independent locus. By further genetic analysis, two SSR and PATE markers were found to tightly link to the avirulence locus. The genetic distance between the marker and the AVR gene was estimated to be 6.1 cM, 13.0 eM respectively. These two markers delimited the Avr-Pid2 in a range of 500 kb in chromosome 7, which provides a solid foundation to clone the kvr gene.

关 键 词：稻瘟病菌 无毒基因 分子标记 

分 类 号：Q75[生物学—分子生物学]