耐药大肠埃希菌插入序列与接合性质粒遗传标记研究  

Investigation of genetic markers of conjuagal plasmids and insertion sequence in drug resistant strains of Escherichia coli

作　　者：黄东标[1] 李嫦珍[1] 陈江平[1] 周茂亮[1] 胡晓燕[1] 黄帮智[1] 

机构地区：[1]浙江省磐安县人民医院检验科,浙江磐安县322300

出　　处：《中华医院感染学杂志》2011年第15期3095-3097,共3页Chinese Journal of Nosocomiology

摘　　要：目的调查耐药大肠埃希菌分离株中插入序列和接合性质粒遗传标记的存在情况。方法收集医院2009年6月-2010年6月临床分离的耐药大肠埃希菌共20株,采用聚合酶链反应(PCR)方法分析4种插入序列:IS26I、S903I、SEcp1I、SCR1和2种接合性质粒遗传标记:traAt、rbC。结果 20株耐药大肠埃希菌共检出3种插入序列:IS26 17株,占85.0%,ISEcp1 12株,占60.0%,IS903 5株,占25.0%;2种接合性质粒遗传标记:traA 15株,占75.0%,trbC 8株,占40.0%,只有ISCR1未检测到。结论同时检测耐药大肠埃希菌的插入序列IS26、IS903I、SEcp1I、SCR1和接合性质粒遗传标记traAt、rbC等基因尚为国内首次报道;菌株对各类抗菌药物耐药率均不低,这可能与插入序列和接合性质粒高携带率相关。OBJECTIVE To investigate the distribution of genetic markers of conjuagal plasmids and insertion sequence in drug resistant strains of Escherichia coli（E.coli）.METHODS From Jun 2009 to Jun 2010,a total of 20 strains of drug resistant E.coli were collected from clinics.Then,4 kinds of insertion sequence（IS26,IS903,ISEcp1,ISCR1） and 2 kinds of conjugal plasmids（traA,trbC） were analyzed by PCR.RESULTS Of 20 strains of E.coli,17 strains（85.0%）,12 strians（60.0%）,5 strains（25.0%）,15 strains（75.0%）,8 strains（40.0%）were detected to carry IS26,ISEcp1,IS903,traA,trbC,respectively.However,ISCR1 was not detected.CONCLUSIONS It′s the first report to detect IS26,IS903,ISEcp1,ISCR1,traA,trbC in China.The fact that high positive rates of genetic markers of conjuagal plasmids and insertion sequence in drug resistant strains might play a role in resistance to antimicrobial agents.

关 键 词：大肠埃希菌 插入序列 接合性质粒 

分 类 号：R378.21[医药卫生—病原生物学]