胫骨癌痛大鼠脊髓背角5-羟色胺水平的变化  

Change in 5-hydroxytryptamine level in spinal dorsal horn in a rat model of tibial bone cancer pain

作　　者：刘希江[1] 曹菲[1] 卜慧莲[1] 高峰[1] 杨辉[1] 田玉科[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院麻醉学教研室,武汉市430030

出　　处：《中华麻醉学杂志》2011年第6期695-698,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30901396,30872441,30700783,30872440）

摘　　要：目的评价胫骨癌痛大鼠脊髓背角5-羟色胺（5-HT）水平的变化。方法雌性SD大鼠60只，体重160～180g，采用随机数字表法，将其随机分为3组（n=20）：对照组（c组）、假手术组（s组）和胫骨癌痛组（P组）。C组不做任何处理；S组于右侧胫骨上段骨髓腔注射D．hank液10μl；P组于右侧胫骨上段骨髓腔接种Walker256大鼠乳腺癌细胞悬液10出制备胫骨癌痛模型。于接种前1d、接种后3、5、7、9、11、14、16、18和21d时测定机械痛阈。于接种前1d、接种后7、14和21d时，每组痛阈测定结束后随机处死大鼠4只，取脊髓组织，采用高效液相色谱法测定脊髓背角5-HT含量；接种后14d取接种侧胫骨组织，光镜下观察胫骨破坏情况。脊髓背角5-HT含量与机械痛阈进行直线相关分析。结果与C组和S组比较，P组接种后7～21d时机械痛阈降低，接种后7、14和21d时脊髓背角5-HT含量升高（P〈0．05），且5-HT含量与机械痛阈呈负相关（r=-0．973，P〈0．05）。C组和S组各时点机械痛阚和脊髓背角5-HT含量比较差异无统计学意义（P〉0．05）。光镜下P组大鼠术侧胫骨骨质严重破坏。结论大鼠胫骨癌痛的形成与维持可能与脊髓背角5-HT水平升高有关。Objective To investigate the change in 5-hydroxytryptomine （5-HT） content in spinal dorsal horn in a rat model of tibial bone cancer pain （ BCP）. Methods Sixty female SD rats weighing 160-180 g were randomly divided into 3 groups （n = 20 each） ： control group （group C）, sham operation group （group S） and BCP group. BCP was induced by intra-tibial inoculation of 10 μl Walker 256 breast cancer cell suspension in group BCP, while group S received intra-tibial inoculation of 10 μl D-hank solution. Paw withdrawal threshold to mechanical stimulation with yon Frey filaments （MWT） was measured 1 d before （baseline） and at 3, 5, 7, 9, 11, 14, 16, 18 and 21 d after breast cancer cell inoculation. At 1 d before and 7, 14 and 21 d after breast cancer cell inoculation, four animals in each group were sacrificed after measurement of MWT. Their lumbar segments of the spinal cord were removed for assay of 5-HT content in spinal dorsal horn using HPLC with fluorescence detector. HE staining was used to detect the damage to the tibia. Correlation between the 5-HT content and MWT was ana- lyzed. Results MWT was significantly decreased after breast cancer cell inoculation in group BCP （ P 〈 0.05 ） . Microscopic examination showed serious bone destruction of tibia at the injection site in group BCP, while no bone destruction was found in groups C and S. 5-HT content in spinal dorsal horn was significantly higher in group BCP than in groups C and S （P 〈 0.05）. There was strong negative linear correlation between 5-HT content in spinal dorsal horn and MWT （ r = - 0.973, P 〈 0.05）. Conclusion The 5-HT content in spinal dorsal horn is significantly increased in rats with tibial BCP and is involved in the development of BCP.

关 键 词：血清素 骨肿瘤 疼痛 脊髓 

分 类 号：R285.5[医药卫生—中药学]