异氟醚麻醉对新生大鼠海马激活肌细胞增强因子2信号通路的影响  

Effects of isoflurane anenthesia on myocyte enhancer factor 2 signaling pathway in neonatal rat hippocampus

作　　者：赵以林[1] 罗爱林[1] 金小高[1] 王金韬[1] 谭蕾[1] 施庆余[1] 李世勇[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院麻醉科,武汉市430030

出　　处：《中华麻醉学杂志》2011年第6期714-716,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30772086）

摘　　要：目的探讨异氟醚麻醉对新生大鼠海马激活肌细胞增强因子2（MEF2）信号通路的影响。方法取出生5d的SD大鼠24只，雌雄不拘，体重10-13g，采用随机数字表法，将大鼠随机分为2组（n=12）：对照组（c组）和异氟醚组（I组）。I组大鼠放置在密闭箱中，吸入1．5％异氟醚和纯氧，吸入时间6h，C组不做任何处理。于异氟醚麻醉2、4、6h和麻醉结束后24h（T1-4）时（C组于相应时点）分别取3只大鼠，断头处死，取海马组织，采用RT-PCR法测定MEF2mRNA、synGAPImRNA和ArcmRNA及突触素ImRNA的表达水平，采用Westrnblot法测定突触素I蛋白的表达水平。结果与C组比较，I组T1-3时海马MEF2mRNA、synGAPImRNA、ArcmRNA和突触素ImRNA、T2-4。时海马突触素I蛋白表达水平均上调（P〈0．05）。结论吸人麻醉浓度的异氟醚可能通过激活新生大鼠海马MEF2信号通路从而影响发育期突触的形成。Objective To investigate the effects of isoflurane anenthesia on myocyte enhancer factor 2 （MElt2） signaling pathway in neonatal rat hippocampus. Methods Twenty-four 5-day-old SD rats of both sexes, weighing 10-13 g, were randomly divided into 2 groups （ n = 12 each） ： control group （group C） and isoflurane group （group I）. In group I, 1.5% isoflurane in 100% 02 was inhaled for 6 h. Group C received no treatment. Three rats in each group were sacrificed at 2, 4, 6 h of isoflurane anenthesia and 24 h after isoflurane anenthesia （T1-4）, and the hippocampi removed for determination of MEF2 mRNA, synGAP I mRNA, Arc mRNA and synapsin I mRNA expression （by PT-PCR） and synapsin I protein expression （by Western blot）. Results Compared with group C, the expression of MEF2 mRNA, synGAP I mRNA, Arc mRNA and synapsin I mRNA at T1-3 and synapsin I protein at T2-4 was up-regulated in group I （ P 〈 0.05）. Conclusion Inhalation of anaesthetic concentration of isoflurane may affect synapse formation during the development of central nervous system by activating hippocampal MEF2 signaling pathways in neonatal rats.

关 键 词：异氟醚 肌形成调节因子 婴儿 新生 海马 

分 类 号：R614[医药卫生—麻醉学]