不同剂量异丙酚对老龄大鼠慢性缺血性脑损伤后认知功能的影响  

Effect of different doses of propofol on cognitive function after chronic cerebral ischemia-induced injury in aged rats

作　　者：陈岗[1] 米卫东[1] 张宏[1] 

机构地区：[1]解放军总医院麻醉手术中心,100853

出　　处：《中华麻醉学杂志》2011年第6期720-722,共3页Chinese Journal of Anesthesiology

摘　　要：目的评价不同剂量异丙酚对老龄大鼠慢性缺血性脑损伤后认知功能的影响。方法雄性SD大鼠80只，月龄18月，体重400-500g，采用随机数字表法，将大鼠随机分为4组（n=20），假手术组（S组）、缺血组（I组）、不同剂量异丙酚组（P1组和P2组）。I组、P1组和P1组采用永久性结扎双侧颈总动脉制备慢性低灌性脑缺血损伤模型，S组仅穿线，不结扎。于术后1d，S组和I组腹腔注射生理盐水2．5ml，P1组和P2组分别腹腔注射异丙酚10、50m/kg，容量2．5ml，2次／d，连续7d。于末次给药后第3天（T1）和第33天（T2）行水迷宫测试和离体海马CAI区长时程增强（LTP）的检测。结果与S组比较，I组、P1组和P2组T1和T2时潜伏期延长，经过原平台次数减少，第4象限游泳时间与总游泳时间比值、LTP诱发率明显降低（P〈0．05）；与I组比较，T1时P1组和P2组潜伏期延长，经过原平台次数减少，第4象限游泳时间与总游泳时间比值和LTP诱发率降低（P〈0．05），T2时P2组潜伏期延长，经过原平台次数减少，第4象限游泳时间与总游泳时间比值和LTP诱发率降低（P〈0．05），P1组上述各项指标差异无统计学意义（P〉0．05）。结论异丙酚在改善大鼠缺血性脑损伤时对认知功能的损害是加重的，高剂量异丙酚作用更明显。Objective To investigate the effects of different doses of propofol on cognitive function after chronic cerebral ischemianduced injury in aged rats. Methods Eighty male SD rats, aged 18 months, weighing 400-500 g, were randomly divided into 4 groups （ n = 20 each） ： shame operation group （group S）, chronic cerebral ischemia group （group I）, two propofol groups （groups Pt and P2 ）. The chronic cerebral ischemia was induced by permanent occlusion of bilateral common carotid arteries. On 1 day after operation, intraperitoneal normal saline 2.5 ml was injected twice a day for 7 consecutive days in groups S and I, and intraperitoneal propofol 10 and 50 mg/kg in 2.5 ml of normal saline were injected twice a day for 7 consecutive days in groups Px and P2 respectively. On 3rd and 33rd days after the last injection （T1,2）, 10 rats in each group underwent Morris water maze test to assess the cognitive function. After the test was completed, the rats were sacrificed and the.hippocampi were removed and sliced （450-500 btm thick）. Schaffer lateral branch in CA1 region was stimulated to induce long-term potentiation （LTP）. Results Compared with group S, the escape latency was significantly prolonged, the number of animals＇ swimming aeross the platform, the ratio of the swimming time spent in the forth quadrant to the total swimming time, and the success rate of LTP induction were significantly decreased at T1 and T2 in groups I, Pt and P2 （P 〈 0.05）. Compared with group I, the escape latency was significantly prolonged, the number of animals＇ swimming across the platform, the ratio of the swimming time spent in the forth quadrant to the total swimming time, and the success rate of LTP induction were significantly decreased at T1 in groups P2 and P2, and at T2 in group P2 （ P 〈 0.05 ）. Conclusion Propofol aggravates the damage to cognitive function while it attenuates the chronic cerebral ischemia-induced injury in aged rats, especially the high dose.

关 键 词：二异丙酚 脑缺血 认知障碍 老年人 

分 类 号：R614[医药卫生—麻醉学]