异丙酚预先给药对大鼠前脑缺血再灌注时海马单核细胞趋化因子1及其受体表达的影响  

Effect of propofoi pretreatment on hippocampal monocyte chemotactic protein.1 and CC-chemokine receptor type 2 expression following forebrain ischemia-reperfusion in rats

作　　者：郭永清[1] 侯晓来[1] 刘友章[1] 张华萍[1] 郭政[2] 

机构地区：[1]山西省人民医院麻醉科,太原市030012 [2]山西医科大学第二医院麻醉科

出　　处：《中华麻醉学杂志》2011年第6期754-756,共3页Chinese Journal of Anesthesiology

摘　　要：目的探讨异丙酚预先给药对大鼠前脑缺血再灌注时海马单核细胞趋化因子1（MCP-1）及其受体（CCR2）表达的影响。方法健康成年雄性SD大鼠24只，体重250-300g，随机分为3组（n=8）：假手术组（S组）仅暴露双侧颈总动脉及股动静脉置管，不夹闭；缺血再灌注组（IR组）采用夹闭双侧颈总动脉10min合并放血降压再回输法制备前脑缺血再灌注损伤模型；异丙酚预先给药组（P组）脑缺血前即刻股静脉注射异丙酚50mg／kg。于再灌注6h时处死大鼠取脑，分离海马神经元，采用RT-PCR法测定MCP-1mRNA和CCR2mRNA的表达，Western blot法测定MCP-1和CCR2蛋白的表达。结果IR组和P组海马神经元MCP-1及CCR2表达较S组上调（P〈0．05）；P组海马神经元MCP-1及CCR2表达较IR组下调（P〈0．05）。结论异丙酚预先给药可抑制海马神经元MCP-1及CCR2表达，该作用可能会减轻大鼠前脑缺血再灌注损伤。Objective To investigate the effect of propofol pretreatment on hippocampal monocyte chemotactic protein-1 （MCP-1） and CC-chemokine receptor type 2 （CCR2） expression following forebrain ischemiareperfusion （I/R） in rats.Methods Twenty-four male SD rats weighing 250-300 g were randomly divided into 3 groups （n = 8 each） ： group Ⅰ control; groupⅡI/R and group Ⅲ propofol pretreatment. Cerebral I/R was induced by clamping bilateral common carotid arteries for 10 min combined with hypotension （MAP was maintained at 35-45 mm Hg） induced by exsanguinations in group Ⅱ and Ⅲ. In groupⅢpropofol 50 mg/kg was injected into femoral vein immediately before cerebral ischemia. The animals were sacrificed at 6 h of reperfusion. Hippocampal tissue was obtained for detection of MCP-1 mRNA and CCR2 mRNA and their protein expression by RT-PCR and Western blot technique. Results I/R significantly increased the expression of MCP-1 and CCR2 in hippocampal tissue as compared with control group. Propofol pretreatment significantly attenuated cerebral I/R induced increase in MCP-1 and CCR2 expression. Conclusion Propofol pretreatment can significantly inhibit forebrain I/R-induced hippocampal MCP-1 and CCR2 expression.

关 键 词：二异丙酚 趋化因子CCL2 受体 CCR2 再灌注损伤 脑 

分 类 号：R965[医药卫生—药理学]