正相高效液相色谱大粒径手性固定相拆分普萘洛尔对映体研究  

Chiral separation of propranolol with normal phase-HPLC and big particle diameter chiral stationary phase

作　　者：武晓玉[1] 王荣[1,2] 杨沛[2] 谢华[1] 马骏[1] 贾正平[1,2] 郭志强[1] 

机构地区：[1]兰州军区兰州总医院临床药理基地,兰州730050 [2]兰州大学药学院,兰州730000

出　　处：《药物分析杂志》2011年第8期1496-1499,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家科技部重大资助项目(No.2008ZXJ09014-010)

摘　　要：目的:建立正相高效液相色谱拆分盐酸普萘洛尔对映异构体的方法。方法:采用自行合成的纤维素衍生物大粒径手性固定相,考察了拆分方法,以正己烷-异丙醇-三乙胺溶液(95∶5∶0.1%)为流动相,流速0.5mL·min-1,检测波长为276nm。结果:盐酸普萘洛尔两对映异构体的保留时间分别为8.67mim、14.36min,分离度良好;日内、日间精密度的RSD均小于3%。加样回收率在95.5%～101.4%之间,RSD为2.4%。结论:结果表明所建立的大粒径纤维素衍生物手性固定相高效液相色谱拆分盐酸普萘洛尔制剂中对映异构体的方法,简便、可靠、高效,可用于盐酸普萘洛尔制剂中对映异构体的测定。Objective：To develop a normal phase-high performance liquid chromatography method for separation of the two enantiomer of propranolol.Methods：The synthesis of chiral stationary phase has been set up by means of cellulose derivatives.The mobile phase was the mixture of n-hexane-isopropanol-triethylamine（95∶ 5∶ 0.1%） with the flow rate of 0.5 mL·min-1,and the detection wavelength was set at 276 nm.Results：The retention time of two enantiomers of propranolol was 8.67 mim and 14.36 min,respectively.Under the optimized conditions,enantiomers of propranolol were well separated from the baseline.The RSD of the inner-day and day to day precision was smaller than 3%.The recoveries was 95.5%-101.4% with RSD of 2.4%.Conclusion：The results show that the method can enantioseparate the two enantiomers of propranolol by HPLC and the chiral stationary phase of big particle diameter cellulose derivative.This separation approach developed is simple,reliable and accurate,and and may be used for routine analysis of enantiomers of the tablet of propranolol.

关 键 词：心得安 普萘洛尔 对映体 手性拆分 高效液相色谱 大粒径 手性固定相 

分 类 号：R917[医药卫生—药物分析学]