Cu(Ⅱ)-阿莫西林体系的荧光特性及Cu(Ⅱ)的含量测定  

The fluorescence characteristic of Cu^(2+)-amoxicillin and the determination of Cu^(2+)

作　　者：龙立平[1] 谌委菊[1] 肖谷清[1] 钟桐生[1] 

机构地区：[1]湖南城市学院化学与环境工程院,益阳413049

出　　处：《中国抗生素杂志》2011年第8期606-609,共4页Chinese Journal of Antibiotics

基　　金：湖南省自然科学基金资助项目(07JJ6068);湖南省科技计划资助项目(2010HJ4123)

摘　　要：目的建立荧光光度法测定实际样品中铜离子的含量。方法基于铜离子对阿莫西林的荧光有显著的熄灭作用,建立了荧光法快速测定铜离子含量的新方法。结果铜离子与阿莫西林发生配合反应,配合物的组成比为1∶2,将该方法用于水样中铜离子的测定,铜离子浓度的线性范围为1.00×10-6~5.00×10-2mol/L,检出限为1.87×10-7mol/L。样品回收率为94.0%~106.5%。结论该方法简便、快速、准确。Objective We established a new method for quantitative determination of Cu^2＋. Method CuCu^2＋could quench the fluorescence intensity of amoxicillin. Based on this reaction, CuCu^2＋ was determined by fluorescence spectrophotometry. Results The cooperate reaction between amoxicillin and CuCu^2＋ took place, the composition of the complex between CuCu^2＋ and amoxicillin was 1：2. The reaction conditions were investigated in detail, The linear range of determination for Cu^2＋ was 1.00 ×10^-6-5.00 × 10^-2 mol/L, with detection limit of 1.87×10^-7mol/L. The recovery of Cu^2＋ was 94.0%-106.5%. Conclusion This method is simple, accurate, rapid and can be used to determination of Cu^2＋ in the water.

关 键 词：荧光光度法 荧光熄灭 阿莫西林 铜(Ⅱ) 

分 类 号：O657.3[理学—分析化学]