耐甲氧西林金黄色葡萄球菌肺炎小鼠感染模型的建立与评估  

Establishment and evaluation of a pneumonia model in mice after infection with an isolated methicillin-resistant Staphylococcus aureus(MRSA)

作　　者：王星[1] 周文江[2] 沈艳[2] 刘芳[2] 杨华[2] 高谦[1] 王德成[1] 

机构地区：[1]教育部/卫生部医学分子病毒学重点实验室复旦大学上海医学院,上海200032 [2]上海市公共卫生临床中心复旦大学附属公共卫生临床中心,上海201508

出　　处：《中国抗生素杂志》2011年第8期617-620,共4页Chinese Journal of Antibiotics

基　　金：上海市科委资助项目(项目编号:07DZ19503)

摘　　要：目的本研究采用临床分离鉴定的ST239型耐甲氧西林金黄葡萄球菌(MRSA)感染BALB/c小鼠,建立小鼠肺炎模型,对小鼠的临床症状、肺载菌量与组织病理学变化进行时相性监测,并用该模型验证万古霉素对小鼠的治疗效果。方法取35只小鼠随机分成MRSA感染组、药物组和对照组,通过滴鼻方式分别滴入50μL细菌(前两组)与PBS(对照组);感染一天后对药物组用同样的方式滴入万古霉素,CT检测肺部的变化,并对肺载菌量和组织病理学变化进行观察。结果与对照组比较,CT和细菌计数等结果表明MRSA感染组和药物组的肺组织有明显的炎症反应,载菌量较高;与感染组相比,药物组小鼠肺部的载菌量明显降低,气管和肺部的组织病理学症状明显减轻。结论本实验结果表明小鼠MRSA肺炎模型成功建立,并可用于药物疗效的比较评估。该模型的建立,将为进一步研究临床分离的MRSA的病原特性、发病机理、治疗方法等,提供可靠的小动物模型。Objective Staphylococcus aureus pneumonias are increasingly recognized as a major form of life- threatening infections. In this study, we established a pneumonia model of BALB/c mice after infection with an ST239 MRSA strain, which was a popular MRSA stain and isolated and identified from Shanghai Huashan hospital. Methods We monitored the clinical signs and gross observations of MRSA-infected mice, and defined the pneumonia with microCT scanning, lung bacteria loading counting and histopathology examination. Results This isolated ST239 MRSA strain can caused a severe injury in the trachea and lung, the obvious symptoms including the desquamated cilia and infiltration of inflammatory cells in the lung. However, the damaged symptoms in respiratory system were improved importantly in the vancomycin-treated mice. Conclusion The solid results obatined in this model will benefit us to study the pathogenic characteristics and patholgenesis in MRSA-induced pneumonia, and propeled us to seek a safety cure approaches in the future.

关 键 词：耐甲氧西林金黄色葡萄球菌 肺炎 小鼠模型 

分 类 号：R378.1[医药卫生—病原生物学]