应用聚合酶链反应在海南检测间日疟原虫的效果  

DETECTION OF PLASMODIUM VIVAX BY POLYMERASE CHAIN REACTION IN HAINAN

作　　者：何建文[1] 蔡贤铮[2] 张永生[1] 王香凤[2] 华德[2] 王赛梅[2] 

机构地区：[1]上海第二军医大学长海医院实验诊断科,上海200433 [2]海南省热带病防治研究所,海口570203

出　　处：《中国寄生虫学与寄生虫病杂志》1999年第6期356-358,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：WHO/TDR部分资助!(No.ID920374)

摘　　要：目的： 建立适合疟疾流行区现场应用的ＰＣＲ 检测间日疟的方法， 并在现场与常规涂片镜检法进行比较。方法： 通过改良血样的采集及模板处理， 设计引物及优化反应条件等， 建立简便、敏感与特异的ＰＣＲ检测间日疟原虫的方法， 并在海南省疟疾流行区对３１０ 例间日疟患者与镜检法进行比较。结果： ＰＣＲ 检测间日疟原虫具有特异性， 其敏感性为１０ 个原虫／μｌ。ＰＣＲ检测和镜检法的阳性率分别为３４．２％ 和３１．９％ ， 与ＰＣＲ比较， 镜检法有５ 例误诊或漏诊。结论： 此ＰＣＲ 法可用于现场检测间日疟患者。AIM: To establish a modified polymerase chain reaction(PCR) method for the detection of Plasmodium vivax in the endemic areas of malaria and compare the method with the conventional light microscopy in the field.METHODS: A PCR method was modified by improving the collection procedures of blood samples, template extraction, primer design and optimizing the reaction condition. The method was evaluated by examining blood samples from 310 patients with vivax malaria and compared with the conventional light microscopy in endemic areas of Hainan Province. RESULTS: The positive rates of the modified PCR method and microscopic method were 34\^2% and 31\^9%, respectively. CONCLUSION: The modified PCR method is simple, sensitive and specific for the detection of vivax malaria patients in endemic areas. \;

关 键 词：间日疟原虫 聚合酶链反应 诊断 疟疾 

分 类 号：R531.304[医药卫生—内科学]