IFN-γ联合TNF-α活化小鼠腹腔巨噬细胞抗不同毒力株弓形虫的作用  

ANTI-TOXOPLASMA EFFECT OF ACTIVATED MOUSE MACROPHAGES INDUCED BY INTERFERON\|γ COMBINED WITH TNF\|α\+*

作　　者：张爱民[1] 杨惠珍[2] 杨杨[2] 钱宗立[2] 

机构地区：[1]上海铁道大学医学院寄生虫学教研室,上海200331 [2]上海第二医科大学寄生虫学教研室,上海200025

出　　处：《中国寄生虫学与寄生虫病杂志》1999年第6期350-352,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学基金!资助项目(No.39470636)

摘　　要：目的： 比较ＩＦＮ－γ联合ＴＮＦ－α体外活化的小鼠腹腔巨噬细胞（ＭΦ） 对强毒株ＲＨ株及弱毒株Ｆｕｋａｙａ株的抗虫作用。方法： 体外以ＩＦＮ－γ与ＴＮＦ－α联合活化昆明系小鼠腹腔ＭΦ，观察其对入侵的ＲＨ株及Ｆｕｋａｙａ株速殖子的抗虫作用， 测定培养上清中ＮＯ的水平。结果： 在以ＩＦＮ－γ１００Ｕ＋ ＴＮＦ－α１００Ｕ 活化的ＭΦ中， 入侵２４ｈ 后的ＲＨ株弓形虫速殖子被完全杀灭， 而Ｆｕｋａｙａ 株速殖子则缓慢增殖， 且前者培养上清中ＮＯ水平显著高于后者。结论：ＩＦＮ－γ活化的ＭΦ对入侵的ＲＨ 株和Ｆｕｋａｙａ株速殖子的抗虫作用存在差异，这可能与ＮＯ的水平有关。AIM: To compare the inhibitory effect of the macrophages activated by IFN\|γ combined with TNF\|α against RH strain and Fukaya strain. METHODS: The average parasite proliferation rates of the two strains within the cytokine\|activated MΦs were calculated at different times post\|challenge, the nitric oxide (NO) levels in the medium supernatant were simultaneously determined. RESULTS: In the macrophages activated by 100 U each of IFN\|γ and TNF\|α, the invaded tachyzoites of RH strain were completely killed, while the invaded tachyzoites of Fukaya strain remained slow proliferation with significantly lower levels of NO detected at 24 h post challenge. CONCLUSION: The difference in the anti\|Toxoplasma effect of the activated macrophages against RH and Fukaya strains might be attributed to the different amount of NO produced by the macrophages.\;

关 键 词：Γ-干扰素 TNF-Α 弓形虫 巨噬细胞 一氧化氮 

分 类 号：R531.805[医药卫生—内科学]