日本血吸虫感染小鼠肝脏及骨髓中IL-4,IL-5和IL-10水平的动态变化  

DYNAMIC CHANGES IN IL\|4, IL\|5 AND IL\|10 IN LIVER AND BONE MARROW OF MICE INFECTED WITH SCHISTOSOMA JAPONICUM\+*

作　　者：曾令兰[1] 罗端德[1] 刘薇[1] 郭劲松[1] 李淑莉[1] 

机构地区：[1]同济医科大学附属协和医院传染病教研室,武汉430022

出　　处：《中国寄生虫学与寄生虫病杂志》1999年第6期377-379,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：湖北省血吸虫病防治委员会基金!资助项目(No.1994-19)

摘　　要：目的： 探讨小鼠感染日本血吸虫后肝脏与骨髓产生Ｔｈ２ 细胞因子的变化。方法： 采用免疫组化测定，并用多媒体病理图文定量分析， 观察感染后第８、１０ 及第１２ 周小鼠肝脏及骨髓ＩＬ－４、ＩＬ－５ 和ＩＬ－１０ 的变化。结果及结论： 在感染小鼠肝脏， ＩＬ－４， ＩＬ－５ 和ＩＬ－１０ 随感染时间的延长而明显升高， 且以ＩＬ－４ 最为明显。而在骨髓，ＩＬ－４随感染时间延长而升高， 但在第１０ 及１２ 周时明显低于肝脏； ＩＬ－５在感染的第１２ 周内明显高于肝脏， ＩＬ－１０在感染的１０ ｗ ｋ 前呈增高趋势，但第１２ 周下降，并明显低于肝脏。小鼠的肝脏是感染日本血吸虫后机体免疫应答的主要场所， 而在感染骨髓中， ＩＬ－５存在明显的自分泌。AIM: To observe the changes in Th2 cytokines in the liver and bone marrow of mice infected with schistosomiasis japonica. METHODS: ABC immunohistochemical staining technique and multimedia pathological picture analysis were used. Meanwhile, IL\|4, IL\|5 and IL\|10 derived from livers and bone marrows of infected mice were observed at wk 8, 10 and 12 after infection. RESULTS AND CONCLUSION: In the livers of infected mice, the levels of IL\|4, IL\|5 and IL\|10 increased obviously with a prolongation of the infection duration, IL\|4 being the highest. However, in the bone marrow of infected mice, IL\|4 level increased slowly with the duration of the infection, being lower than those in the liver at wk 10 and 12 after infection. The level of IL\|5 was higher than that in the liver within 12 wk. At wk 10 after infection IL\|10 tended to increase, but decreased thereafter and was obviously lower than that in the liver. In mice infected with Schistosoma japonicum, the immune response occurred mainly in the liver. IL\|5 in the bone marrow showed auto\|secretion. \;

关 键 词：日本血吸虫病 白细胞介素4 IL-5 IL-10 

分 类 号：R532.21[医药卫生—内科学]