降钙素基因相关肽基因转导对内皮损伤兔髂动脉血管环舒缩反应的影响  

The effect of calcitonin gene-related peptide gene transduction on contraction and relaxation in the artery rings of rabbit iliac artery injury model

作　　者：金晓峰[1] 徐成斌[2] 张彤[2] 崔俊峰[2] 王申五[3] 

机构地区：[1]北京邮电总医院心内科,100032 [2]北京医科大学人民医院心血管内科,北京100044 [3]北京医科大学人民医院中心实验室,北京100044

出　　处：《中国病理生理杂志》1999年第12期1074-1077,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助!( No .39570310)

摘　　要：目的：探讨降钙素基因相关肽（ ＣＧＲＰ） 基因转导兔动脉内膜对其舒缩功能的影响。方法：利用逆转录病毒载体，将ＣＧＲＰ 基因在体转导兔髂动脉内膜，取动脉环做灌流试验，研究其对血管活性物质的反应。结果：转基因血管内皮细胞的增殖较对照组活跃，动脉的内皮依赖性舒张反应较强，１０ －６ｍｏｌ／Ｌ 的乙酰胆碱引起的舒张为３３－０８ ％ ±１０－０１ ％ ，对照组为－ １７－５７ ％ ±５－５９ ％ ，Ｐ＜ ０－０１ 。结论：ＣＧＲＰ 基因转导损伤的免髂动脉内膜可促进内皮细胞增殖，并使动脉内皮依赖性舒张反应改善。AIM: To study the effect of calcitonin gene related peptide (CGRP) on contraction and relaxation of iliac artery transduced with CGRP gene by retrovirus vector. METHODS:Use retrovirus vector, the CGRP gene was transduced in the intima of the artery in vivo . Then the responses of artery to phenylephrine acetylcholine (Ach), sodium nitroprusside and norepinephrine were studied.RESULTS:Proliferation of endothelial cells in the artery transduced by CGRP gene was accelerated and endothelium dependent relaxation of the artery was improved, percentage of relaxation arisen by 10 6 mol/L Ach was 33.08%±10.00%,while in the control group it was -17 57%±5.59%, P <0.01 CONCLUSION:Transduction of CGRP gene can promote proliferation of endothelial cells and improve the function of endothelial cells in artery with injured intima.

关 键 词：动脉舒缩功能 动脉内皮损伤 CGRP 

分 类 号：R543.502[医药卫生—心血管疾病]