丹槐银屑浓缩丸对豚鼠银屑病样模型组织VEGF、VEGFR含量的影响  

Effects of Danhuai Yinxie Concentrated Pill on VEGF and VEGFR in Psoriasis-like Guinea Pigs' Cutaneous Lesions

作　　者：王学军[1] 胡二为[1] 陶以成[2] 

机构地区：[1]黑龙江省中医研究院,哈尔滨150006 [2]中国中医科学院,北京100700

出　　处：《中国中西医结合皮肤性病学杂志》2011年第2期96-98,共3页Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine

基　　金：辽宁省教育厅创新团队项目(2008T026);中国医师协会皮肤科医师分会-宝洁基金

摘　　要：目的通过丹槐银屑浓缩丸对豚鼠银屑病样模型耳部组织血管内皮生长因子(vascular endothelial growthfactor,VEGF)及其受体(vascular endothelial growth factor receptor,VEGFR)水平影响的实验研究,探讨丹槐银屑浓缩丸治疗银屑病的作用机制。方法用5%普萘洛尔乳剂外涂豚鼠耳背制备银屑病样病理改变模型,设置空白组、模型组、西药对照组、成药对照组及高、中、低剂量治疗组。采用双抗体夹心酶联免疫法测定组织VEGF,VEGFR含量,观察其与皮损表现的关系。结果丹槐银屑浓缩丸高、中、低剂量组VEGF水平下降不明显(P>0.05),VEGFR水平出现不同程度下降,特别是中剂量组与其它组差异显著(P<0.01或P<0.05)。结论丹槐银屑浓缩丸可使组织VEGFR水平显著降低,进而抑制血管内皮细胞增生,改善豚鼠银屑病样模型的皮损变化。Objective To investigate the effect of Danhuai Yinxie concentrated pill（DYCP） on the level of vascular endothelial growth factor（VEGF）and its receptor vascular endothelial growth factor receptor（VEGFR） in psoriasis-like guinea pigs＇ cutaneous lesions,so as to explore the mechanism of DYCP＇s treatment for psoriasis.Methods The guinea pigs were made into models of psoriasis by external application of 5%propranolol cream at skin of ears and then divided into blank group,model group,western medicine group（control group）,Chinese patient medicine group（control group）,DYCP high,medium and low dose group（treatment group）.The contents of VEGF and VEGFR in cutaneous lesions were detected by enzyme-linked immuno sorbent assay（ELISA）,then study the relation between the contents of above detection and the manifestation of cutaneous lesions.Results DYCP high,medium and low dose group showed a higher level,having no statistical difference with all the other groups（P0.05）.The level of VEGFR was declined to different degrees,especially for the medium dose group（P0.01 or P0.05）.Conclusion DYCP could significantly reduce the level of VEGFR in cutaneous lesions,and then inhibit the proliferation of endothelial cells and improve cutaneous lesions of psoriasis-like guinea pigs.

关 键 词：丹槐银屑浓缩丸 银屑病 VEGF VEGFR 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]