乌鳢和斑鳢微卫星制备及其对自交与杂交F1代的初步鉴定  

Isolation of Microsatellite Makers for Channa argus and C. maculate and Primary Identification of C. argus, C. maculate and Their Reciprocal Hybrids

作　　者：朱树人[1] 李忠全[2] 冯晓宇[2] 谢楠[2] 朱林[1] 李家乐[1,3] 

机构地区：[1]上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海201306 [2]杭州市农业科学研究院,杭州310024 [3]上海市高校水产养殖学E研究院,上海201306

出　　处：《动物学杂志》2011年第4期1-7,共7页Chinese Journal of Zoology

基　　金：杭州市科技局重大科技创新项目(No.20083212A04);上海市水产养殖重点学科建设项目(No.Y1101)

摘　　要：通过磁珠富集法分离了乌鳢(Channa argus)和斑鳢(C.maculate)的微卫星DNA序列,根据这些微卫星DNA序列的两翼序列,采用Primer 3.0或Primer 5.0软件设计出乌鳢与斑鳢的微卫星引物各31对。用62对微卫星引物对乌鳢和斑鳢自交与杂交F1代的4个组合进行扩增,10对引物无扩增产物,其余52对引物中,均能在4个组合的鱼中扩增出条带的引物有49对。位点CHA16在乌鳢中呈多态,在斑鳢中呈单态;位点CHM23在乌鳢中呈单态,在斑鳢中呈现多态;另外,位点CHM24在乌鳢中呈单态,在斑鳢中没有扩增出条带。检测到乌鳢和斑鳢的差异位点22个,其中18个均可单独有效鉴定出F1子代的乌鳢、斑鳢及包括正交和反交在内的杂交鳢,其余4个位点也可以通过组合鉴定出F1子代的乌鳢、斑鳢及杂交鳢。但是,杂交鳢中正交组合和反交组合不能通过本研究开发的微卫星鉴别。In the present study,microsatellite DNA was isolated and enriched from the genomes of Channa argus and C.maculate by magnetic beads.Sixty-two pairs of microsatellite primers of C.argus and C.maculate were designed based on the flanking sequence of the microsatellite loci with software Primer 3.0 or Primer 5.0.Ten primer pairs failed to amplify.Among the 52 primer pairs which could effectively amplify products,49 universal primer pairs could effectively amplify in F1 generation.Loci CHA16 showed monomorphic in C.maculate and polymorphic in C.argus and loci CHM23 was opposite.In addition,CHM24 showed monomorphic in C.argus and failed to show in C.maculae.Moreover,22 specific loci between C.argus and C.maculate were screened out,and 18 specific loci could identify C.argus,C.maculate and the reciprocal hybrids alone effectively.Combination of the other 4 specific loci also effectively identified C.argus,C.maculate and the reciprocal hybrids.However,the reciprocal hybrids [C.argus（♀）×C.maculata（♂）,C.maculata（♀）×C.argus（♂）]could not be identified in this research.

关 键 词：乌鳢 斑鳢 杂交子代 微卫星 鉴定 

分 类 号：S917.4[农业科学—水产科学]