异氟醚预处理对缺血再灌注损伤后幼兔心肌TNF-α和iNOS mRNA表达的影响及其机制  

Effects of isoflurane preconditioning on TNF-α and iNOS mRNA expressions in ischemia reperfusion myocardium of young rabbits and mechanisms

作　　者：刘国利[1] 沈途[1] 张锦英[1] 

机构地区：[1]辽宁医学院附属第一医院麻醉科,辽宁锦州121001

出　　处：《吉林大学学报（医学版）》2011年第4期622-625,773,共5页Journal of Jilin University：Medicine Edition

基　　金：辽宁省教育厅科研基金资助课题(2008400)

摘　　要：目的:观察异氟醚(ISO)预处理对幼兔缺血再灌注心肌组织中肿瘤坏死因子-α(TNF-α)和诱导型一氧化氮合酶(iNOS)mRNA表达的影响,并阐明其作用机制。方法:取纯种新西兰幼兔36只,随机分为3组:假手术组只穿线不结扎;缺血再灌注(I/R)组,结扎30min,再灌注2h;ISO预处理组,缺血前吸入1.1%异氟醚30min,洗脱15min后处理同I/R组。缺血30min再灌注2h后,用放射免疫法检测心肌组织中TNF-α的含量和原位杂交法检测心肌组织中iNOS mRNA表达。结果:ISO组和I/R组幼兔心肌TNF-α含量升高,与假手术组比较差异有统计学意义(P<0.05);而ISO组与I/R组比较,心肌TNF-α含量降低,差异有统计学意义(P<0.05)。ISO组和I/R组心肌组织中iNOS mRNA的表达水平明显高于假手术组(P<0.05);ISO组与I/R组比较,iNOS mRNA表达水平明显下降(P<0.05)。结论:异氟醚通过减少TNF-α的生成和iNOS mRNA的表达以减轻I/R损伤而发挥保护作用。Objective To investigate the effects of isoflurane（ISO） preconditioning on the expressions of TNF-α and iNOS mRNA in ischemia reperfusion myocardium of young rabbits and clarify their mechanisms.Methods 36 young rabbits were randomly divided into three groups,the rabbits in sham operation group were not occluded coronary artery;the rabbits in ischemia-reperfusion（I/R） group were occluded for 30 min and reperfused for 2 h;the rabbits in ISO preconditioning group were inhaled 1.1% isoflurane for 30 min before ischemia,others were the same to I/R group after 15 min.The content of myocardial TNF-α and the expression of iNOS mRNA in myocardioum were determined 30 min after coronary occlusion and 2 h after reperfusion.Results Compared with sham operation group,the contents of myocardial TNF-α in I/R and ISO groups were significantly increased（P〈0.05）;the content of myocardial TNF-α in ISO group was significantly lower than that in I/R group（P〈0.05）.The expressions of iNOS mRNA in I/R and ISO groups were significantly higher than that in sham operation group（P〈0.05）,the expression of iNOS mRNA in ISO group was significantly lower than that in I/R group（P〈0.05）.Conclusion ISO could reduce the TNF-α content and the expression of iNOS mRNA during I/R injury,which may be one of molecular mechanisms of ISO preconditioning in myocardial protection.

关 键 词：异氟醚 缺血再灌注 肿瘤坏死因子-α 诱导型一氧化氮合酶 

分 类 号：R614.2[医药卫生—麻醉学]