HIV-1母婴传播早期诊断检测方法应用  

Early laboratory detection of HIV type 1 in infants

作　　者：贺健梅[1] 陈曦[1] 李向忠[1] 张国强[1] 江洋[1] 邹潇白[1] 丁莉莎[1] 

机构地区：[1]湖南省疾病预防控制中心,湖南长沙410005

出　　处：《中国病毒病杂志》2011年第4期279-281,共3页Chinese Journal of Viral Diseases

基　　金：全球基金中国艾滋病防治项目(CHN-304-G03-H);湖南省卫生厅科研计划项目(B2010-091)

摘　　要：目的应用核酸扩增技术对母婴传播婴幼儿人类免疫缺陷病毒（HIV）感染进行早期诊断,为临床应用提供依据。方法对2009-2010年HIV阳性孕产妇活产的34例3-14个月龄婴幼儿收集足底干血斑样本进行HIV-1 DNA检测。随访18个月后进行血清HIV抗体检测比较。结果 34例婴幼儿中,检出HIV-1 DNA 9例,检出率26.5%。9例HIV-1 DNA阳性患儿均未使用过抗病毒治疗。18个月后随访,34例婴幼儿除1例失访,2例检出HIV-1 DNA婴儿已死亡（根据临床有机会性感染体征诊断为AIDS）,7例检出HIV-1 DNA婴儿血清抗体阳性外,其他24例未检出HIV-1 DNA的婴儿其血清抗体为阴性。两种方法检测结果比较符合率为100.0%。结论核酸检测是有效的早期诊断方法,早期诊断有利于后续开展母婴阻断工作。Objective To establish and evaluate polymerase chain reaction for the early detection of HIV type 1 in infants.Methods A total of 34 dried blood spot（DBS） samples were collected during 2009 and 2010 from 3-14 months infants of HIV positive mothers.All the specimens were tested by qualitative HIV-1 DNA polymerase chain reaction.HIV antibodies were tested with Western blot assay after 18 months following-up.Results HIV-1 DNA was positive in 9（26.5%） samples by HIV-1 DNA polymerase chain reaction.Of 9 HIV-1 DNA positive infants,7 were HIV antibodies positive and 2 died of AIDS related opportunity infection after 18 months follow-up.There was no anti-HIV positive conversion among 25 HIV-1 DNA negative infants.Conclusions HIV-1 DNA polymerase chain reaction is a sensitive assay for the early detection of HIV-1 infection among newborns and infants.It should be helpful for the prevention of mother-to-child transmission of HIV.

关 键 词：母婴传播 HIV-1DNA检测 蛋白印迹试验 HIV抗体 干血斑样本 

分 类 号：R512.91[医药卫生—内科学]