源于克雷伯氏菌Klebsiella sp. AC-11的3株噬菌体的生物学特性  

Biological characteristics of 3 lytic bacteriophages from the same host Klebsiella sp. AC-11

作　　者：刘斌[1,2] JOST Gunter SCHMIEL Juliane WOLF Arite 黄翔玲[1,2] 谷力[1,2] 王玉桥[1,2] 叶德赞[1,2] 

机构地区：[1]国家海洋局第三海洋研究所,福建厦门361005 [2]国家海洋局海洋生物遗传资源重点实验室,福建厦门361005 [3]莱布尼兹波罗的海研究所,德国罗斯托克D-18119 [4]罗斯托克大学分子生理与生物技术实验室,德国罗斯托克D-18051

出　　处：《海洋学报》2011年第4期147-154,共8页

基　　金：中-德合作项目:“不同水环境中土著噬菌体与其宿主细菌共存系统的研究”(HB080302(1))

摘　　要：从国家海洋局第三海洋研究所海水养殖池中分离得到一株细菌AC-11,16SrDNA序列分析表明其与肺炎克雷伯氏菌肺炎亚种(Klebsiella pneumoniaesub sp. pneumoniae)具有最高的序列同源性达99.78%。以此菌株为宿主菌,于2008年11月采用双层平板法分离得到3株裂解性噬菌体KP1,KP2和KP3,其中KP2分离自同一海水养殖池,另外2株则来源于厦门筼筜湖海水样品。分别对宿主菌及噬菌体进行了电镜形态观察,并对噬菌体进行了BglⅡ和EcoRⅤ两种酶的限制性酶切分析,发现它们均属于长尾噬菌体科(Siphoviridae),但具有不同的酶切图谱;同时测定了3株噬菌体的最佳感染复数、一步生长曲线及对温度、pH、紫外线、乙醚和氯仿等理化因素的敏感性等生理特性。实验结果表明3株噬菌体具有不同的生物学特性,为不同株型。本研究丰富了对噬菌体多样性的认识,并为进一步应用噬菌体诊断和治疗潜在的由肺炎克雷伯氏菌引发的疾病及细菌分型等研究提供了理论基础。The bacterial strain AC-11 was isolated from marine aquaculture pond of the Third Institute of Oceanography in Xiamen,China,and its 16SrDNA sequence demonstrated 99.78% similarity to Klebsiella pneumoniae subsp.pneumoniae.A total of 3 lytic phages KP1,KP2 and KP3 were isolated with this bacterial strain as the host using double-layer agar method.Phage KP2 was from the same aquaculture pond where the host strain was isolated,and the other two phages were from Yuan Dang Lake（marine water） in Xiamen,China.Morphology of both phages and host were observed under transmission electron microscope.The restriction patterns of nucleic acid of the phages were estimated by dsDNA restriction enzyme BglⅡand EcoRⅤ.All of the 3 phages belonged to family Siphoviridae,but with different genome fingerprint patterns.The physiological characteristics of the phages such as the optimal multiplicity of infection,the one-step growth curves and the sensitivity to temperature,pH,UV light,ether and chloroform were also tested.The results indicated that the phages had different biological characteristics and thus belonged to different strain types.This could contribute to the diversity of phages,the diagnosis and treatment of potential diseases caused by Klebsiella pneumoniae and typing of the relating Klebsiella bacteria.

关 键 词：肺炎克雷伯氏菌肺炎亚种(Klebsiella PNEUMONIAE sub sp. pneumoniae) 噬菌体 电子显微镜观察 生物学特性 

分 类 号：Q93[生物学—微生物学]