电针结合中频对腰神经根受压模型大鼠抗炎镇痛的作用  

Experimental study on anti-inflammatory and analgesic effects of electroacupuncture combined with medium frequency therapy in model rats with lumbar nerve root compression

作　　者：朱峰[1] 艾炳蔚[2] 高建芸[1] 

机构地区：[1]东南大学附属中大医院针灸科,江苏南京210009 [2]江苏省中医院针灸科

出　　处：《中国针灸》2011年第8期721-726,共6页Chinese Acupuncture & Moxibustion

基　　金：江苏省中医药管理局课题资助项目:HZ07023

摘　　要：目的:探讨电针结合中频治疗腰神经根受压的效应及作用机制。方法:将72只SD大鼠随机分为正常组、假手术组、模型组、电针组、中频组和电针加中频组,每组12只。后4组经手术复制腰神经根受压模型。正常组、假手术组、模型组不做任何治疗,电针组进行电针治疗,穴取"夹脊""环跳"等,中频组采用中频治疗,穴取"夹脊""环跳",电针加中频组先后进行电针与中频治疗,取穴及治疗时间与上两组相同。所有治疗均于造模成功后第5天开始,每日1次,连续治疗14 d。观察治疗前后大鼠患肢大体观,检测治疗后血浆血栓素B 2(TXB 2)、前列环素F1α(PGF1α)含量,并通过光镜观察受压神经根局部的病理变化。结果:与治疗前相比,3个治疗组均能明显降低模型大鼠患肢神经功能评分(均P<0.01)。治疗后,与模型组相比,电针组及电针加中频组TXB 2下降明显(均P<0.01),电针加中频组PGF1α上升明显(P<0.01),3组的TXB 2/PGF1α比值均得到了良性调整(均P<0.01),同时电针组、电针加中频组的病理评分改善明显(均P<0.01)。结论:电针结合中频治疗对腰神经根受压模型大鼠具有抗炎镇痛的作用;降低TXB 2、升高PGF1α、维持两者的动态平衡,从而改善微循环状态是其作用机制之一。Objective To explore the effects and mechanism of electroacupuncture combined with medium frequency therapy on lumbar nerve root compression. Methods Seventy-two Sprague-Dawley （SD） rats were randomly divided into a normal group, a sham operation group, a model group, an eleetroacupuncture group（EA group）, a medium frequency group（MF group） and an electroacupuncture combined with medium frequency group （EA＋ MF group）, twelve rats in each group. Models were established by surgery except the normal group and the sham operation group. Rats in the normal group, the sham operation group and the model group were not treated. In the EA group, the rats were treated by electroacupuncture at ＂Jiaji＂ （EX-B 2） and ＂Huantiao＂ （GB 30） etc.. and by medium frequency at the ＂Jiaji＂（EX-B 2） and ＂Huantiao＂（GB 30） in the MF group. Rats in the EA--MF group were treated by both electroacupuncture and medium frequency. All treatments were started on the fifth day of established model, once a day for fourteen days. Rats＇ lower limb functions were observed before and after treatment, thromhoxane B 2 （TXB 2） and prostacyclin F1α （PGF1α） in blood plasma were tested after treatment, and pathological changes in the local compressed nerve root were observed by light microscope. Results After treatment, the scores of rats＇ lower limb neurologic function in three therapy groups were significantly lower than before （all P〈0.01）. Compared with the model group, TXB 2 in the EA group and the EA＋MF group after treatment were decreased significantly （both P〈0.01）. and PGFla in the EA＋MF group was increased significantly （P〈0.01）. and TXB 2/PGF1α level were all regulated favorably in three therapy groups（all P〈0.01）. and the pathological scores in the EA group and the EA＋MF group were improved significantly （both P〉0.01）. Conclusion Electroacupuncture combined with medium frequency has anti-inflammatory and analgesic effects m model rats with lumbar ne

关 键 词：腰神经根受压 模型 大鼠 电针 中频 血栓素B2 前列环素F1α 

分 类 号：R245.9[医药卫生—针灸推拿学]