Iscom佐剂增强含前S表位重组乙肝表面抗原的免疫原性  

Iscom matrix enhances the immunogenicity of hepatitis B virus surface antigen containing PreS epitopes

作　　者：谭昌耀 叶琳 蒋丽明 张鹏艳 胡波 Bror Morein 

机构地区：[1]成都生物制品研究所生物技术研究室,610023 [2]瑞典农业科学大学兽医微生物学系病毒学部

出　　处：《中华微生物学和免疫学杂志》2011年第7期643-646,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的探讨Iscom佐剂对含前S表位重组乙肝表面抗原（SS1S2）免疫原性的影响。方法用纯化的SSIS2抗原与A1（OH），和Iscom佐剂分别配制疫苗，在0d和14d时采用肌肉和皮下注射方式免疫BALB／c小鼠，免后14d各取-半小鼠采血，测定乙肝病毒s、前s1和前s2抗体滴度以及总的IgG1和IgG2a抗体滴度，并计算IgG2a和IgG1抗体比例。同时分离脾淋巴细胞，进行IFN-1酶联斑点（ELISPOT）测定。结果单针免疫时，两组疫苗血清样品乙肝病毒S、前S1和前S2抗体阳转率、抗体滴度及IFN-1分泌细胞数相当，但Iscom佐剂疫苗组IgG2a抗体比例较高；加强免疫后，两组疫苗抗体滴度均呈上升趋势，Iscom疫苗组抗体滴度上升幅度大于AI（OH），疫苗组，两者S、前S1和前s2抗体滴度差异均有统计学意义。加强免疫后Iscom疫苗组IgG2a抗体比例保持平衡，而A1（OH），疫苗组IgG2a抗体比例进-步降低。在细胞免疫方面，Iscom疫苗组在加强免疫后产生的特异性IFN-γ分泌细胞数显著超过AI（OH），疫苗组。结论本研究初步显示，Iscom佐剂对含前S表位重组乙肝表面抗原具有比Al（OH），佐剂更强地免疫增强作用。Objective To investigate the effect of Iscom matrix on the immunogenicity of recombinant hepatitis B surface antigen containing PreS epitopes（SS1S2）. Methods SS1S2＋ AI（OH）3 and SS1S2 ＋Iscom vaccines were made by combining purified SSIS2 antigen with AI（OH） 3 adjuvant or Iscom matrix. Groups of BALB/c mice were injected i. m or s. c by either of the two vaccines at day 0 and day 14. Half of the mice were sacrificed and sera were taken and spleen ceils separated from the mice 14 days after each injection. Anti-S, anti-PreS1, and anti-PreS2 antibody titers were measured, and total IgG1 and IgG2a titers were further detected for each serum sample. IFN-γ ELISPOT assay was performed to detect IFN-3, secreting cells from the pooled spleen cells for each vaccine group. Results The seroconversion rates and geometric mean titers （GMTs） and the numbers of IFN-γ secreting cells were approximately at the same level for the differently formulated vaccines after the first injection except that the ratio of IgG2a to IgG1 in the Iseom group was higher than the AI（ OH）3 group. After boost injection, the GMTs of total IgG rise slightly in the AI（ OH）3 group but significantly in the Iscom group. The IgG2a to IgG1 ratio in the Iscgm group kept balanced while dropped further in the A1 （OH）3 group. The number of speeific IFN-γ secreting cells triggered by the Iscom vaccine exceeded significantly the number of AI（OH） 3 vaccine, showing a stronger cellular response. Conclusion The results in this study shows that Iscom matrix is more potent in enhancing the immunogenieity of recombinant hepatitis B virus surface antigen containing PreS epitopes than A1 （OH） 3 adjuvant.

关 键 词：ISCOM 前S 表位 乙肝表面抗原 免疫原性 

分 类 号：R392[医药卫生—免疫学]